A Negative Regulatory Element Controls mRNA Abundance of the Leishmania mexicana Paraflagellar Rod Gene PFR2
A Negative Regulatory Element Controls mRNA Abundance of the Leishmania mexicana Paraflagellar Rod Gene PFR2
复制标题
负调控元件控制墨西哥利什曼原虫副鞭毛杆基因 PFR2 的 mRNA 丰度
作者：
K. K. Mishra;T. Holzer;L. Moore;J. Lebowitz
摘要墨西哥利什曼原虫PFR2基因座编码一种鞭毛旁杆（PFR）的组成部分，这种鞭毛结构只存在于生活史的昆虫阶段。PFR2 mRNA水平在哺乳动物阶段比在昆虫阶段低10倍。核运行实验表明，PFR2 mRNA丰度的变化是在转录后实现的。对PFR2C的整个1，400个核苷酸的3′非翻译区（UTR）的缺失和阻断取代分析导致鉴定出包含在3′ UTR的10个核苷酸内的调节元件，称为PFR调节元件（PRE），其对于PFR2 mRNA水平的10倍调节是必需的。比较PFR2转录物的半衰期，除了存在或不存在的PRE，相同的，揭示了PRE的作用是通过破坏无鞭毛体中PFR2 mRNA的稳定。将PRE插入指导嵌合PFR2转录物的组成型表达的构建体中。PRE的插入导致该转录物的调节表达，表明该调节元件足以用于前鞭毛体特异性表达。由于PRE存在于所有L. mexicana PFR基因检测到目前为止，我们建议它作为一种手段协调PFR基因的表达。
ABSTRACT The Leishmania mexicana PFR2 locus encodes a component of the paraflagellar rod (PFR), a flagellar structure found only in the insect stage of the life cycle. PFR2 mRNA levels are 10-fold lower in the mammalian stage than in the insect stage. Nuclear run-on experiments indicate that the change in PFR2 mRNA abundance is achieved posttranscriptionally. Deletion and block substitution analysis of the entire 1,400-nucleotide 3′ untranslated region (UTR) of PFR2C led to the identification of a regulatory element contained within 10 nucleotides of the 3′ UTR, termed the PFR regulatory element (PRE), that is necessary for the 10-fold regulation of PFR2 mRNA levels. Comparison of the half-lives of PFR2 transcripts, identical except for the presence or absence of the PRE, revealed that the PRE acts by destabilizing the PFR2 mRNA in amastigotes. The PRE was inserted into a construct which directs the constitutive expression of a chimeric PFR2 transcript. Insertion of the PRE resulted in regulated expression of this transcript, demonstrating that the regulatory element is sufficient for promastigote-specific expression. Since the PRE is present in the 3′ UTR of all L. mexicana PFR genes examined so far, we propose that it serves a means of coordinating expression of PFR genes.
酿酒酵母中 ARE 介导的 mRNA 衰减的调节。
影响因子：
16
作者：
Vasudevan,S;Peltz,SW
通讯作者：
Peltz,SW
影响因子：
10.5
作者：
LEBOWITZ, JH;SMITH, HQ;BEVERLEY, SM
通讯作者：
BEVERLEY, SM
克氏锥虫表面抗原 FL-160 的表达受 3 非翻译区、3 基因间区和编码区中的元件控制。
影响因子：
1.5
作者：
Weston,D;LaFlamme,AC;VanVoorhis,WC
通讯作者：
VanVoorhis,WC