Ectopic expression of the apple nucleus-encoded thylakoid protein MdY3IP1 triggers early-flowering and enhanced salt-tolerance in Arabidopsis thaliana.
Ectopic expression of the apple nucleus-encoded thylakoid protein MdY3IP1 triggers early-flowering and enhanced salt-tolerance in Arabidopsis thaliana.
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苹果核编码的类囊体蛋白 MdY3IP1 的异位表达引发拟南芥提早开花并增强耐盐性
DOI：
10.1186/s12870-018-1232-6
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发表时间：
2018-01-20
影响因子：
5.3
通讯作者：
Hao YJ
中科院分区：
文献类型：
--
作者：
Yu JQ;Wang JH;Sun CH;Zhang QY;Hu DG;Hao YJ
背景：植物细胞核编码的类囊体蛋白Y3IP1与质体编码的Ycf3蛋白相互作用，在光系统I（PSI）组装中起重要作用。结果：克隆了苹果MdY3IP1基因，该基因编码的蛋白与拟南芥Y3IP1（AtY3IP1）高度同源。MdY3IP1的异位表达引发了拟南芥植物的早花和耐盐性增强。MdY3IP1通过加速植物细胞内糖代谢过程来调控成花转变，从而影响成花相关基因的表达。MdY3IP1表达植物的耐盐性增加与活性氧（ROS）积累减少，并通过调节生长素的生物合成和运输增加侧根发育，其次是拟南芥耐盐性的增强。总之，这些研究结果提供了新的证据，额外的功能Y3IP1样蛋白和其潜在的机制，其中Y3IP1赋予植物早花和耐盐phenotypes.Conclusions：这些观察结果表明，植物生长和抗逆性可以通过MdY3IP1基因的调节。进一步的分子和遗传学方法将加速我们对MdY3IP1在PSI复合物形成和植物抗逆性中功能的了解，并为创造早熟和对不利环境条件具有高抗性的转基因作物品种提供策略。
Background:The roles in photosystem I (PSI) assembly of the nucleus-encoded thylakoid protein Y3IP1 who interacts with the plastid-encoded Ycf3 protein that has been well-characterized in plants. However, its function and potential mechanisms in other aspects remain poorly understood.Results:We identified the apple MdY3IP1 gene, which encodes a protein highly homologous to the Arabidopsis Y3IP1 (AtY3IP1). Ectopic expression of MdY3IP1 triggered early-flowering and enhanced salt tolerance in Arabidopsis plants. MdY3IP1 controlled floral transition by accelerating sugar metabolism process in plant cells, thereby influencing the expression of flowering-associated genes. The increase in salt stress tolerance in MdY3IP1-expressing plants correlated with reduced reactive oxygen species (ROS) accumulation, and an increase in lateral root development by regulating both auxin biosynthesis and transport, as followed by enhancement of salt tolerance in Arabidopsis. Overall, these findings provide new evidences for additional functions of Y3IP1-like proteins and their underlying mechanisms of which Y3IP1 confers early-flowering and salt tolerance phenotypes in plants.Conclusions:These observations suggest that plant growth and stress resistance can be affected by the regulation of the MdY3IP1 gene. Further molecular and genetic approaches will accelerate our knowledge of MdY3IP1 functions in PSI complex formation and plants stress resistance, and inform strategies for creating transgenic crop varieties with early maturity and high-resistant to adverse environmental conditions.
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影响因子：
5.6
作者：
Bolouri Moghaddam MR;Van den Ende W
通讯作者：
Van den Ende W
影响因子：
11.6
作者：
Casimiro, I;Marchant, A;Bennett, M
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Bennett, M
影响因子：
7.2
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Clough, SJ;Bent, AF
通讯作者：
Bent, AF
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2.9
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Kirchhoff, H;Mukherjee, U;Galla, HJ
通讯作者：
Galla, HJ
影响因子：
64.8
作者：
Amunts, Alexey;Drory, Omri;Nelson, Nathan
通讯作者：
Nelson, Nathan