Role of Long Noncoding RNA 799 in the Metastasis of Cervical Cancer through Upregulation of TBL1XR1 Expression.
Role of Long Noncoding RNA 799 in the Metastasis of Cervical Cancer through Upregulation of TBL1XR1 Expression.
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长非编码 RNA 799 通过上调 TBL1XR1 表达在宫颈癌转移中的作用
DOI：
10.1016/j.omtn.2018.10.007
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发表时间：
2018-12-07
期刊：
影响因子：
--
通讯作者：
Huang L
中科院分区：
文献类型：
--
作者：
Liao LM;Zhang FH;Yao GJ;Ai SF;Zheng M;Huang L
长链非编码rna （Long noncoding RNAs, lncRNAs）与癌症发生的分子机制密切相关，研究其在宫颈癌中的表达及其机制也具有重要意义。本研究对lncRNA799在宫颈癌中的表达进行研究，以确定其在宫颈癌进展中的临床意义。研究了lncRNA799在218例宫颈癌标本中的表达。lncRNA799在宫颈癌组织中的表达明显高于癌旁正常组织。lncRNA799的过表达与FIGO分期、SCC-Ag水平、淋巴转移有显著相关性，并与较差的生存率相关。lncRNA799的异位表达促进了SiHa细胞的转移，而lncRNA799的敲低对转移有抑制作用。Western blot分析表明，lncRNA799促进转导β样蛋白1相关蛋白（TBL1XR1）的表达，lncRNA799与TBL1XR1在宫颈癌中的表达有较强的相关性。此外，lncRNA799通过作为miR-454-3P的竞争性内源性RNA （ceRNA）调节TBL1XR1的表达。结果提示lncRNA799可作为宫颈癌预后的新标志物。因此，靶向涉及lncRNA799的ceRNA网络可能是一种潜在的治疗宫颈癌的策略。
Long noncoding RNAs (lncRNAs) are closely associated with the molecular mechanisms underlying cancer development, and it would be highly useful to study their expression and mechanisms in cervical cancer too. The current study investigated lncRNA799 expression in cervical cancer in order to determine its clinical importance in the progression of cervical cancer. lncRNA799 expression was studied in 218 cervical cancer samples. Expression of lncRNA799 was significantly higher in the cervical cancer tissue than in the adjacent normal tissue. Overexpression of lncRNA799 was found to have a significant correlation with FIGO stage, SCC-Ag level, and lymphatic metastasis, and it was also associated with poor survival. Ectopic expression of lncRNA799 promoted the metastasis of SiHa cells, whereas lncRNA799 knockdown had an inhibitory effect on metastasis. Western blot analysis demonstrated that lncRNA799 promotes the expression of transducing β-like protein 1-related protein (TBL1XR1), and that lncRNA799 and TBL1XR1 expression show strong correlation in cervical cancer. Moreover, lncRNA799 modulated the expression of TBL1XR1 by acting as a competitive endogenous RNA (ceRNA) for miR-454-3P. The results indicate that lncRNA799 could be used as a novel marker of cervical cancer prognosis. Thus, targeting the ceRNA network involving lncRNA799 could be a potential treatment strategy against cervical cancer.
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