Probing neural codes with two-photon holographic optogenetics.
Probing neural codes with two-photon holographic optogenetics.
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DOI：
10.1038/s41593-021-00902-9
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发表时间：
2021-10
影响因子：
25
通讯作者：
Abdeladim, Lamiae
中科院分区：
文献类型：
--
作者：
Adesnik, Hillel;Abdeladim, Lamiae
光遗传学引发了神经科学家研究大脑功能的一场革命。由于技术限制，大多数光遗传学研究都采用低空间分辨率激活方案，限制了可能进行的扰动类型。然而，更精细的空间尺度上的神经活动操作对于更全面地理解神经计算可能很重要。空间精确的多光子全息光遗传学有望解决这一挑战，并开辟许多以前不可能的新实验类别。更具体地说，通过提供在功能定义的神经元集合中在空间和时间上重建极其特定的神经活动模式的能力，多光子全息光遗传学可以让神经科学家揭示感觉、认知和行为的神经编码的基本方面，这是遥不可及的。这篇综述总结了多光子全息光遗传学的最新进展，这些进展大大扩展了其能力，强调了突出的技术挑战，并概述了它可以执行的实验类别，以测试和验证大脑功能的关键理论模型。多光子全息光遗传学可以帮助关闭实验和理论神经科学之间的循环，从而显着加快神经科学发现的步伐，从而对神经系统功能和疾病产生根本性的新见解。
Optogenetics ushered in a revolution in how neuroscientists interrogate brain function. Due to technical limitations, the majority of optogenetic studies have employed low spatial resolution activation schemes that limit the types of perturbations that could be made. Yet, neural activity manipulations at finer spatial scales are likely to be important to more fully understand neural computation. Spatially precise multiphoton holographic optogenetics promises to address this challenge and opens up many new classes of experiments that were not previously possible. More specifically, by offering the ability to recreate extremely specific neural activity patterns in both space and time in functionally defined ensembles of neurons, multiphoton holographic optogenetics could allow neuroscientists to reveal fundamental aspects of the neural codes for sensation, cognition, and behavior that have been beyond reach. This review summarizes recent advances in multiphoton holographic optogenetics that substantially expand its capabilities, highlights outstanding technical challenges, and provides an overview of the classes of experiments it can execute in order to test and validate key theoretical models of brain function. Multiphoton holographic optogenetics could significantly accelerate the pace of neuroscience discovery by helping to close the loop between experimental and theoretical neuroscience, leading to fundamental new insights into nervous system function and disorder.
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影响因子：
7.7
作者：
Antonini A;Sattin A;Moroni M;Bovetti S;Moretti C;Succol F;Forli A;Vecchia D;Rajamanickam VP;Bertoncini A;Panzeri S;Liberale C;Fellin T
通讯作者：
Fellin T
影响因子：
5.3
作者：
Chaigneau E;Ronzitti E;Gajowa MA;Soler-Llavina GJ;Tanese D;Brureau AY;Papagiakoumou E;Zeng H;Emiliani V
通讯作者：
Emiliani V
影响因子：
7.7
作者：
Baker, Christopher A.;Elyadat, Yishai M.;Bolton, M. McLean
通讯作者：
Bolton, M. McLean
影响因子：
25
作者：
Chan KY;Jang MJ;Yoo BB;Greenbaum A;Ravi N;Wu WL;Sánchez-Guardado L;Lois C;Mazmanian SK;Deverman BE;Gradinaru V
通讯作者：
Gradinaru V
DOI：
10.1126/science.1252367
发表时间：
2014-04-25
期刊：
Science (New York, N.Y.)
影响因子：
--
作者：
Berndt A;Lee SY;Ramakrishnan C;Deisseroth K
通讯作者：
Deisseroth K