Characterization of induced pluripotent stem cell-derived megakaryocyte lysates for potential regenerative applications.
Characterization of induced pluripotent stem cell-derived megakaryocyte lysates for potential regenerative applications.
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DOI：
10.1111/jcmm.13698
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发表时间：
2018-09
影响因子：
5.3
通讯作者：
Figueiredo C
中科院分区：
文献类型：
--
作者：
Baigger A;Eicke D;Yuzefovych Y;Pogozhykh D;Blasczyk R;Figueiredo C
最近，裂解物中存在的血小板衍生生长因子在再生医学领域引起了极大的兴趣，例如伤口愈合以及在无异源细胞培养系统中作为胎牛血清的替代品。然而，这种血小板裂解物的产生完全取决于血小板供体的可用性。在这项研究中，研究了使用源自诱导多能干细胞 (iPSC) 的体外生成的巨核细胞作为典型血小板生长因子的细胞来源的可能性。因此，这些因子的存在和水平是在体外产生的巨核细胞中表征的。与血小板相比，体外生成的巨核细胞显示典型血小板生长因子的转录物和蛋白质水平成倍增加，包括血小板衍生生长因子（PDGF）、转化生长因子（TGF）-1β、血管内皮细胞因子（VEGF）-A、表皮生长因子（EGF）、胰岛素样生长因子（IGF）-1和组织因子（TF）。因此，iPSC 衍生的巨核细胞可以作为一种有效的细胞来源，用于不依赖供体生成富含生长因子的裂解物，在创新细胞培养系统和再生疗法中具有广泛的应用潜力。
Recently, platelet‐derived growth factors present in lysates became an extreme interest in the field of regenerative medicine such as in wound healing and as substitutes to foetal bovine serum in xeno‐free cell culture systems. However, the generation of such platelet lysates completely depends on the availability of platelet donors. In this study, the possibility to use in vitro‐generated megakaryocytes derived from induced pluripotent stem cells (iPSCs) as a cell source for typical platelet growth factors was investigated. Therefore, the presence and levels of those factors were characterized in in vitro‐produced megakaryocytes. In comparison with platelets, in vitro‐generated megakaryocytes showed a multifold increased content in transcript and protein levels of typical platelet growth factors including platelet‐derived growth factors (PDGFs), transforming growth factor (TGF)‐1β, vascular endothelial cell factor (VEGF)‐A, epidermal growth factor (EGF), insulin‐like growth factor (IGF)‐1 and tissue factor (TF). Hence, iPSC‐derived megakaryocytes may serve as an efficient cell source for a donor‐independent generation of growth factor‐rich lysates with a broad application potential in innovative cell culture systems and regenerative therapies.
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影响因子：
7.5
作者：
Pogozhykh O;Pogozhykh D;Neehus AL;Hoffmann A;Blasczyk R;Müller T
通讯作者：
Müller T
DOI：
10.1083/jcb.201006102
发表时间：
2010-11-15
期刊：
The Journal of cell biology
影响因子：
--
作者：
Thon JN;Montalvo A;Patel-Hett S;Devine MT;Richardson JL;Ehrlicher A;Larson MK;Hoffmeister K;Hartwig JH;Italiano JE Jr
通讯作者：
Italiano JE Jr
影响因子：
4.8
作者：
Pezzotta, S.;Del Fante, C.;Antoniazzi, E.
通讯作者：
Antoniazzi, E.
DOI：
10.3109/00365513.2015.1099723
发表时间：
2016-02-17
影响因子：
2.1
作者：
Juhl, Morten;Tratwal, Josefine;Haack-Sorensen, Mandana
通讯作者：
Haack-Sorensen, Mandana
影响因子：
2.8
作者：
Burnouf, Pierre-Alain;Juan, Po-Kai;Burnouf, Thierry
通讯作者：
Burnouf, Thierry