The Mechanism of Antimicrobial Activity of Conjugated Bile Acids against Lactic Acid Bacilli.
The Mechanism of Antimicrobial Activity of Conjugated Bile Acids against Lactic Acid Bacilli.
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DOI：
10.3390/microorganisms11071823
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发表时间：
2023-07-17
期刊：
影响因子：
4.5
通讯作者：
Guo, Chun-Feng
中科院分区：
文献类型：
--
作者：
Chai, Li-Na;Wu, Hua;Wang, Xue-Jiao;He, Li-Juan;Guo, Chun-Feng
关键词：
结合胆汁酸对乳酸杆菌菌株的抗菌活性的机制尚不清楚。本研究的目的是研究两种典型的结合胆汁酸（甘鹅去氧胆酸和牛磺鹅去氧胆酸）在人类小肠生理 pH 值下对四种不同种类乳酸杆菌的抗菌活性机制。分别使用荧光探针 SYTO 9 加碘化丙啶、3,3'-二丙基硫二碳花青碘化物和 5(6)-羧基荧光素二乙酸酯 N-琥珀酰亚胺酯检查细菌细胞膜完整性、跨膜电位和跨膜 pH 梯度。通过基于萤火虫荧光素酶的生物发光方法测量细胞内 ATP 水平。结果发现，结合胆汁酸对乳酸杆菌菌株的抗菌活性具有菌株特异性，甘鹅去氧胆酸对乳酸杆菌菌株的抗菌活性明显高于牛磺鹅去氧胆酸。结合胆汁酸通过破坏膜完整性、耗散跨膜电位、降低跨膜 pH 梯度和消耗细胞内 ATP 来抑制乳酸杆菌菌株的生长。总之，结合胆汁酸对乳酸杆菌的抗菌活性是一个多因素现象。这项研究将为制定提高乳酸杆菌体内耐受胆汁能力的策略提供有价值的信息。
The mechanism underlying antimicrobial activity of conjugated bile acids against strains of lactic acid bacilli is not well understood. The purpose of this study was to investigate two typical conjugated bile acids (glycochenodeoxycholic acid and taurochenodeoxycholic acid) for their mechanisms of antimicrobial activity against four strains of different species of lactic acid bacilli at the physiological pH of the small intestine of humans. The bacterial cell membrane integrity, transmembrane potential, and transmembrane pH gradient were examined using the fluorescence probes SYTO 9 plus propidium iodide, 3,3′-dipropylthiadicarbocyanine iodide, and 5(6)-carboxyfluorescein diacetate N-succinimidyl ester, respectively. The intracellular ATP levels were measured by the firefly luciferase-based bioluminescence method. It was found that the antimicrobial activity of conjugated bile acids against the strains of lactic acid bacilli is strain-specific, and glycochenodeoxycholic acid showed significantly greater antimicrobial activity than taurochenodeoxycholic acid against the strains of lactic acid bacilli. The conjugated bile acids inhibited the growth of strains of lactic acid bacilli by disrupting membrane integrity, dissipating transmembrane potential, reducing the transmembrane pH gradient, and depleting intracellular ATP. In conclusion, the antimicrobial activity of conjugated bile acids against lactic acid bacilli is a multifactorial phenomenon. This study will provide valuable information for developing strategies to improve the ability of lactic acid bacilli to tolerate bile in vivo.
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影响因子：
2.8
作者：
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通讯作者：
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