Localization of the Abelson murine leukemia virus protein in a detergent-insoluble subcellular matrix: architecture of the protein
Localization of the Abelson murine leukemia virus protein in a detergent-insoluble subcellular matrix: architecture of the protein
复制标题
阿贝尔森鼠白血病病毒蛋白在去污剂不溶性亚细胞基质中的定位：蛋白质的结构
作者：
M. Boss;G. Dreyfuss;D. Baltimore
我们用非离子洗涤剂Triton X-100提取Abelson小鼠白血病病毒蛋白P120与其他细胞成分的相互作用。在淋巴系和成纤维细胞系中，大多数Abelson鼠白血病病毒P120相关的激酶活性都存在于洗涤剂不溶的基质中。在细胞短暂暴露于[35S]-蛋氨酸期间，标记的P120主要分布在不溶于洗涤剂的基质(淋巴样细胞)或均匀分布于不溶于洗涤剂的基质和可溶部分(成纤维细胞)。稳态标记的P120均匀分布于两部分(淋巴样细胞)或主要分布于可溶性部分(成纤维细胞)。因此，P120明显地从不溶于洗涤剂的基质转移到可溶于洗涤剂的部分，并伴随着酶活性的丧失。当不溶于洗涤剂的基质与[32P]ATP原位孵育时，观察到P120酪氨酸残基的磷酸化。我们用洗涤剂提取成纤维细胞后，发现了一条8万分子量的P120片段(命名为F80)。在提取的淋巴样细胞中未发现F80，但在提取前将标记的淋巴样细胞和未标记的成纤维细胞混合后产生该片段。F80含有P120的GAG决定簇，但不与Abelson特异性血清反应。这些数据使我们能够将蛋白质的各种特征分配给P120分子的区域，并将Abelson特异的抗原决定簇定位于分子的C-末端区域。
We examined the interaction of Abelson murine leukemia virus protein P120 with other cellular components after extraction with the nonionic detergent Triton X-100. Most of the Abelson murine leukemia virus P120-associated kinase activity was found in the detergent-insoluble matrix in both lymphoid and fibroblast cell lines. The P120 labeled during a short exposure of cells to [35S]-methionine was mainly in the detergent-insoluble matrix (lymphoid cells) or equally distributed in the detergent-insoluble matrix and the soluble fraction (fibroblasts). Steady-state-labeled P120 was distributed equally in the two fractions (lymphoid cells) or mostly in the soluble portion (fibroblasts). Thus, there was an apparent movement of P120 from the detergent-insoluble matrix to the detergent-soluble fraction and a concomitant loss of enzymatic activity. When the detergent-insoluble matrix was incubated with [32P]ATP in situ, phosphorylation of tyrosine residues of P120 was observed. We found an 80,000-molecular-weight fragment of P120 (designated F80) after extraction of fibroblast cells with detergent. F80 was not found in extracted lymphoid cells, but mixing labeled lymphoid cells and unlabeled fibroblasts before extraction produced the fragment. F80 contained the gag determinants of P120 but did not react with Abelson-specific serum. These data allowed us to assign various features of the protein to regions of the P120 molecule and to localize the Abelson-specific antigenic determinants to the C-terminal region of the molecule.
与禽肉瘤病毒 PRCII 的 p105 相关的酪氨酸特异性蛋白激酶活性。
影响因子：
3.7
作者：
Neil,JC;Ghysdael,J;Vogt,PK
通讯作者：
Vogt,PK