SARS-CoV-2 Aptasensors Based on Electrochemical Impedance Spectroscopy and Low-Cost Gold Electrode Substrates.
SARS-CoV-2 Aptasensors Based on Electrochemical Impedance Spectroscopy and Low-Cost Gold Electrode Substrates.
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DOI：
10.1021/acs.analchem.1c04456
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发表时间：
2022-02-01
影响因子：
7.4
通讯作者：
Corrigan DK
中科院分区：
文献类型：
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作者：
Lasserre P;Balansethupathy B;Vezza VJ;Butterworth A;Macdonald A;Blair EO;McAteer L;Hannah S;Ward AC;Hoskisson PA;Longmuir A;Setford S;Farmer ECW;Murphy ME;Flynn H;Corrigan DK
SARS-CoV-2诊断实践广泛涉及基于定量聚合酶链反应（qPCR）的病毒序列核酸扩增或基于抗原的检测，如侧流检测（LFA）。逆转录酶-qPCR可以检测病毒RNA，是灵敏度的金标准。然而，该技术是耗时的，需要昂贵的实验室基础设施和训练有素的工作人员。LFA成本较低，接近真实的时间，并且因为它们是基于抗原的，所以它们具有提供疾病状态的更准确指示的潜力。然而，LFA据报道具有低的现实世界的敏感性，在大多数情况下，只是定性的。在这里，提出了一种基于抗原的电化学适体传感器，它有可能解决这些不足之处。一种针对SARS-CoV-2刺突蛋白的适体被固定在一种低成本的涂金聚酯基底上，这种基底是从血糖测试行业改造而来的。SARS-CoV-2的临床相关检测水平是以简单、无标记的测量形式实现的，使用鼻咽拭子样本的样本孵育时间短至15分钟。该测定可容易地针对大规模生产进行优化，并且与低成本仪表兼容。
SARS-CoV-2 diagnostic practices broadly involve either quantitative polymerase chain reaction (qPCR)-based nucleic amplification of viral sequences or antigen-based tests such as lateral flow assays (LFAs). Reverse transcriptase-qPCR can detect viral RNA and is the gold standard for sensitivity. However, the technique is time-consuming and requires expensive laboratory infrastructure and trained staff. LFAs are lower in cost and near real time, and because they are antigen-based, they have the potential to provide a more accurate indication of a disease state. However, LFAs are reported to have low real-world sensitivity and in most cases are only qualitative. Here, an antigen-based electrochemical aptamer sensor is presented, which has the potential to address some of these shortfalls. An aptamer, raised to the SARS-CoV-2 spike protein, was immobilized on a low-cost gold-coated polyester substrate adapted from the blood glucose testing industry. Clinically relevant detection levels for SARS-CoV-2 are achieved in a simple, label-free measurement format using sample incubation times as short as 15 min on nasopharyngeal swab samples. This assay can readily be optimized for mass manufacture and is compatible with a low-cost meter.
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DOI：
10.3390/molecules21040421
发表时间：
2016-03-28
期刊：
Molecules (Basel, Switzerland)
影响因子：
--
作者：
Hasegawa H;Savory N;Abe K;Ikebukuro K
通讯作者：
Ikebukuro K
影响因子：
9.4
作者：
Lee RA;Herigon JC;Benedetti A;Pollock NR;Denkinger CM
通讯作者：
Denkinger CM
影响因子：
6.1
作者：
Maugi, Rushabh;Gamble, Bernadette;Platt, Mark
通讯作者：
Platt, Mark
SARS-CoV-2 感染的诊断。
影响因子：
41.2
作者：
Kevadiya BD;Machhi J;Herskovitz J;Oleynikov MD;Blomberg WR;Bajwa N;Soni D;Das S;Hasan M;Patel M;Senan AM;Gorantla S;McMillan J;Edagwa B;Eisenberg R;Gurumurthy CB;Reid SPM;Punyadeera C;Chang L;Gendelman HE
通讯作者：
Gendelman HE
利物浦无症状测试试验中的Innova SARS-COV-2抗原快速横向流程测试：基于人群的同类研究。
DOI：
10.1136/bmj.n1637
发表时间：
2021-07-06
期刊：
BMJ (Clinical research ed.)
影响因子：
--
作者：
García-Fiñana M;Hughes DM;Cheyne CP;Burnside G;Stockbridge M;Fowler TA;Fowler VL;Wilcox MH;Semple MG;Buchan I
通讯作者：
Buchan I