Peptides corresponding to the predicted heptad repeat 2 domain of the feline coronavirus spike protein are potent inhibitors of viral infection.
Peptides corresponding to the predicted heptad repeat 2 domain of the feline coronavirus spike protein are potent inhibitors of viral infection.
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DOI：
10.1371/journal.pone.0082081
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发表时间：
2013
期刊：
影响因子：
3.7
通讯作者：
Chueh LL
中科院分区：
文献类型：
--
作者：
Liu IJ;Tsai WT;Hsieh LE;Chueh LL
猫传染性腹膜炎（FIP）是由猫冠状病毒（FCoV）引起的一种致死性免疫介导疾病。目前，没有有效的治疗方法。在寻找可能证明临床上有效对抗FCoV感染的药物时，基于FCoV刺突蛋白的推定的七肽重复2（HR 2）序列设计并合成了五个类似的重叠肽，并评估了抗病毒效力。 本研究采用空斑减少试验和MTT（3-（4，5-二甲基噻唑-2-基）-2，5-二苯基四唑溴化物）细胞毒性试验。使用空斑减少测定选择肽以抑制猫冠状病毒感染。 结果表明，浓度低于20 μM的肽（FP 5）可抑制高达97%的病毒复制。将表现出最有效的抗病毒效果的肽（FP 5）进一步与已知的抗病毒剂人干扰素-α（IFN-α）组合，并且观察到显著的协同抗病毒效果。 我们的数据表明，合成肽FP 5可以作为一个有价值的除了目前的FIP预防方法。
Feline infectious peritonitis (FIP) is a lethal immune-mediated disease caused by feline coronavirus (FCoV). Currently, no therapy with proven efficacy is available. In searching for agents that may prove clinically effective against FCoV infection, five analogous overlapping peptides were designed and synthesized based on the putative heptad repeat 2 (HR2) sequence of the spike protein of FCoV, and the antiviral efficacy was evaluated. Plaque reduction assay and MTT (3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide) cytotoxicity assay were performed in this study. Peptides were selected using a plaque reduction assay to inhibit Feline coronavirus infection. The results demonstrated that peptide (FP5) at concentrations below 20 μM inhibited viral replication by up to 97%. The peptide (FP5) exhibiting the most effective antiviral effect was further combined with a known anti-viral agent, human interferon-α (IFN-α), and a significant synergistic antiviral effect was observed. Our data suggest that the synthetic peptide FP5 could serve as a valuable addition to the current FIP prevention methods.
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影响因子：
3.8
作者：
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DOI：
10.1074/jbc.m110.207084
发表时间：
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期刊：
The Journal of biological chemistry
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