Micromanipulation of prophase I chromosomes from mouse spermatocytes reveals high stiffness and gel-like chromatin organization.
Micromanipulation of prophase I chromosomes from mouse spermatocytes reveals high stiffness and gel-like chromatin organization.
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对小鼠精母细胞早期I期染色体的显微操作显示出高硬度和凝胶样染色质结构。
DOI：
10.1038/s42003-020-01265-w
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发表时间：
2020-09-30
影响因子：
5.9
通讯作者：
Qiao H
中科院分区：
文献类型：
--
作者：
Biggs RJ;Liu N;Peng Y;Marko JF;Qiao H
在有性生殖有机体中，减数分裂在连续两次分裂后产生四个单倍体细胞。减数分裂过程中的一个关键事件是联会复合体(SC)的构建，这是一座以蛋白质为基础的大桥，物理上连接着同源染色体。SC有助于减数分裂重组、染色体紧凑，并最终在中期I分离同源染色体。我们提供了直接测量捕获的哺乳动物减数分裂前期I染色体的物理性质的实验。小鼠的减数分裂染色体比体细胞有丝分裂染色体坚硬约十倍，即使是缺乏SC的中心元件SYCP1的遗传突变也是如此。减数分裂染色体在核酸酶作用下溶解，但在蛋白酶作用下才减弱，这表明SC不是刚性连接的，减数分裂前期I染色体是染色质的凝胶网络，类似于有丝分裂染色体。这些结果与液晶或液晶SC是一致的，但SC染色质的硬度足以机械地驱动交叉干扰。Ronald Biggs等人。报道从小鼠精母细胞中分离的完整染色体的生物物理测量。他们将减数分裂前期I的染色体与有丝分裂染色体进行比较，发现减数分裂染色体要坚硬得多，这种坚硬并不依赖于联会复合体的中心元件(SYCP1)。
Meiosis produces four haploid cells after two successive divisions in sexually reproducing organisms. A critical event during meiosis is construction of the synaptonemal complex (SC), a large, protein-based bridge that physically links homologous chromosomes. The SC facilitates meiotic recombination, chromosome compaction, and the eventual separation of homologous chromosomes at metaphase I. We present experiments directly measuring physical properties of captured mammalian meiotic prophase I chromosomes. Mouse meiotic chromosomes are about ten-fold stiffer than somatic mitotic chromosomes, even for genetic mutants lacking SYCP1, the central element of the SC. Meiotic chromosomes dissolve when treated with nucleases, but only weaken when treated with proteases, suggesting that the SC is not rigidly connected, and that meiotic prophase I chromosomes are a gel meshwork of chromatin, similar to mitotic chromosomes. These results are consistent with a liquid- or liquid-crystal SC, but with SC-chromatin stiff enough to mechanically drive crossover interference. Ronald Biggs et al. report biophysical measurements of intact chromosomes isolated from mouse spermatocytes. They compare chromosomes in meiosis prophase I to mitotic chromosomes and find that meiotic chromosomes are much stiffer, and this stiffness does not depend on the central element of the synaptonemal complex (SYCP1).
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影响因子：
4.5
作者：
Qiao H;Chen JK;Reynolds A;Höög C;Paddy M;Hunter N
通讯作者：
Hunter N
影响因子：
3.3
作者：
Poirier, MG;Eroglu, S;Marko, JF
通讯作者：
Marko, JF
DOI：
10.1083/jcb.200910085
发表时间：
2010-03-08
期刊：
The Journal of cell biology
影响因子：
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作者：
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通讯作者：
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影响因子：
4.5
作者：
Pattabiraman D;Roelens B;Woglar A;Villeneuve AM
通讯作者：
Villeneuve AM
DOI：
10.1007/978-3-319-58592-5_20
发表时间：
2017-01-01
期刊：
CENTROMERES AND KINETOCHORES: DISCOVERING THE MOLECULAR MECHANISMS UNDERLYING CHROMOSOME INHERITANCE
影响因子：
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作者：
Mirkovic, Mihailo;Oliveira, Raquel A.
通讯作者：
Oliveira, Raquel A.