Cell behavior on lithographically defined nanostructured substrates
Cell behavior on lithographically defined nanostructured substrates
复制标题
光刻定义的纳米结构基底上的细胞行为
DOI：
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发表时间：
2003
期刊：
影响因子：
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通讯作者：
P. Nealey
中科院分区：
文献类型：
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作者：
A. Teixeira;G. Abrams;C. Murphy;P. Nealey
光刻定义的衬底通过允许呈现受控的细胞刺激，为细胞行为的研究提供了独特的机会。我们使用带有明确尺寸的凹槽和脊状图案的基质，研究了基质地形对人类角膜上皮细胞行为的影响。在每个基板上，我们包括了从微米到纳米级的特征尺寸。这项工作的动机是这样一个事实，即体内上皮组织下的表面，即基底膜，具有丰富的具有纳米级特征的地形。我们发现，细胞通过与图案方向对齐来响应小到70纳米宽的地形特征。此外，底物的地形影响细胞的内部组织，导致细胞骨架元素(肌动蛋白细丝)和粘连结构(局部粘连)的排列。因此，具有与要素相同长度比例尺的要素尺寸的合成地形…
Lithographically defined substrates offer unique opportunities for the study of cell behaviors, by allowing the presentation of controlled cell stimuli. We have investigated the effects of substrate topography on the behavior of human corneal epithelial cells using substrates patterned with grooves and ridges of well-defined dimensions. On each substrate, we included feature sizes ranging from the micrometer to the nanometer scale. This work was motivated by the fact that the surface that underlies epithelial tissues in vivo, the basement membrane, has a rich topography with features of nanoscale dimensions. We found that cells responded to topographic features as small as 70 nm wide by aligning with the pattern direction. Additionally, substrate topography affected internal organization of the cell, inducing the alignment of cytoskeletal elements (actin filaments) and adhesive structures (focal adhesions). Therefore, synthetic topographies with feature dimensions of the same length scale as the features ...
影响因子：
56.9
作者：
Han, J;Craighead, HG
通讯作者：
Craighead, HG