Changes in ionizing radiation dose rate affect cell cycle progression in adipose derived stem cells.
Changes in ionizing radiation dose rate affect cell cycle progression in adipose derived stem cells.
复制标题
电离辐射剂量率的变化影响脂肪干细胞的细胞周期进程。
DOI：
10.1371/journal.pone.0250160
复制
发表时间：
2021
期刊：
影响因子：
3.7
通讯作者：
Dean D
中科院分区：
文献类型：
--
作者：
Rusin M;Ghobrial N;Takacs E;Willey JS;Dean D
辐射的生物医学应用被用于有效的诊断和治疗工具，但可能会给健康组织带来风险。用于诊断目的的高能光子具有很高的穿透深度，可以根据不同材料的衰减特性区分多个组织。同样，在肿瘤内的不同靶点储存能量的能力使利用光子治疗癌症变得有效。聚焦于肿瘤的辐射会在它与生物结构(如DNA)相互作用时沉积能量，如果组织的修复能力被淹没，这将导致细胞死亡。同样，对正常的非癌症组织的损害是辐射的结果，可能导致急性或晚期的慢性毒性特征。脂肪来源的干细胞(ADSCs)是一种间充质干细胞，已被证明具有与骨髓来源的干细胞相似的特征，只是它们更容易获得。在体内，ADSCs起免疫调节剂的作用，协助组织的维护和修复。它们已被证明具有出色的分化能力，使其成为干细胞治疗和再生医学应用的一个极其可行的选择。由于ADSC来源的组织，它们极有可能受到放射治疗的影响，特别是在治疗局部ADSC含量相对较高的结构的肿瘤时(如乳腺癌)。出于这个原因，这项研究背后的目的是更好地了解ADSCs如何受到相当于放射治疗的一小部分的辐射剂量的影响。我们还测量了ADSCs对不同剂量率暴露的反应，以确定ADSCs对放射治疗相关剂量的电离辐射的反应是否存在显着差异。我们的研究结果表明，2GY的中等剂量铯(Cs，137)-伽马射线和低剂量率(1.40Gy/min)或高剂量率(7.31Gymin)的照射均可使ADSCs的增殖率减慢，并在某些人群中出现细胞周期停滞。ADSCs的这些反应并不像以前在其他干细胞类型中测量的那样明显。此外，我们的结果表明，在小动物或细胞照射平台中通常使用的剂量率范围的不同对ADSCs的功能影响最小。ADSCs在再生医学领域的潜力使其成为研究电离辐射的理想候选细胞，因为它们是促进组织愈合的主要细胞类型之一。
Biomedical use of radiation is utilized in effective diagnostic and treatment tools, yet can introduce risks to healthy tissues. High energy photons used for diagnostic purposes have high penetration depth and can discriminate multiple tissues based on attenuation properties of different materials. Likewise, the ability to deposit energy at various targets within tumors make the use of photons effective treatment for cancer. Radiation focused on a tumor will deposit energy when it interacts with a biological structure (e.g. DNA), which will result in cell kill should repair capacity of the tissue be overwhelmed. Likewise, damage to normal, non-cancerous tissues is a consequence of radiation that can lead to acute or late, chronic toxicity profiles. Adipose derived stem cells (ADSCs) are mesenchymal stem cells that have been proven to have similar characteristics to bone marrow derived stem cells, except that they are much easier to obtain. Within the body, ADSCs act as immunomodulators and assist with the maintenance and repair of tissues. They have been shown to have excellent differentiation capability, making them an extremely viable option for stem cell therapies and regenerative medicine applications. Due to the tissue ADSCs are derived from, they are highly likely to be affected by radiation therapy, especially when treating tumors localized to structures with relatively high ADSC content (eg., breast cancer). For this reason, the purpose behind this research is to better understand how ADSCs are affected by doses of radiation comparable to a single fraction of radiation therapy. We also measured the response of ADSCs to exposure at different dose rates to determine if there is a significant difference in the response of ADSCs to radiation therapy relevant doses of ionizing radiation. Our findings indicate that ADSCs exposed to Cesium (Cs 137)-gamma rays at a moderate dose of 2Gy and either a low dose rate (1.40Gy/min) or a high dose rate (7.31Gy/min) slow proliferation rate, and with cell cycle arrest in some populations. These responses ADSCs were not as marked as previously measured in other stem cell types. In addition, our results indicate that differences in dose rate in the Gy/min range typically utilized in small animal or cell irradiation platforms have a minimal effect on the function of ADSCs. The potential ADSCs have in the space of regenerative medicine makes them an ideal candidate for study with ionizing radiation, as they are one of the main cell types to promote tissue healing.
登录
查看更多内容
影响因子：
--
作者：
Berrington de González A;Mahesh M;Kim KP;Bhargavan M;Lewis R;Mettler F;Land C
通讯作者：
Land C
DOI：
10.1016/j.jacr.2007.03.002
发表时间：
2007-05-01
期刊：
Journal of the American College of Radiology : JACR
影响因子：
--
作者：
Amis, E Stephen Jr;Butler, Priscilla F;Zeman, Robert K
通讯作者：
Zeman, Robert K
影响因子：
3.3
作者：
Zuk, PA;Zhu, M;Hedrick, MH
通讯作者：
Hedrick, MH
影响因子：
5.7
作者：
Rodningen, AK;Overgaard, J;Borresen-Dale, AL
通讯作者：
Borresen-Dale, AL
影响因子：
0.9
作者：
Azimian H;Bahreyni-Toossi MT;Rezaei AR;Rafatpanah H;Hamzehloei T;Fardid R
通讯作者：
Fardid R