Requirements for the catalytic cycle of the N-ethylmaleimide-Sensitive Factor (NSF).
Requirements for the catalytic cycle of the N-ethylmaleimide-Sensitive Factor (NSF).
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DOI：
10.1016/j.bbamcr.2011.06.003
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发表时间：
2012-01
期刊：
影响因子：
--
通讯作者：
Whiteheart SW
中科院分区：
文献类型：
--
作者：
Zhao C;Smith EC;Whiteheart SW
N-乙基马来酰亚胺敏感因子（NSF）是与各种细胞活性相关的ATP酶（AAA+）家族的最初成员之一。在这篇综述中，我们讨论了什么是已知的NSF行动的机制，以及如何涉及到其他AAA+蛋白的机制。与其他家族成员一样，NSF与蛋白质复合物结合（即，SNAP-SNARE复合物），并利用ATP水解来影响该复合物的构象。SNAP-SNARE复合物的分解对于SNARE的回收和持续的膜运输是必不可少的。NSF是同源六聚体;每个原聚体由N端结构域NSF-N和两个相邻的AAA结构域NSF-D1和NSF-D2组成。突变分析已经确定了NSF-D1（催化ATP酶结构域）和NSF-N（SNAP-SNARE结合结构域）的许多结构元件的特定作用。用（Ni 2 +-NTA）2-Cy 3标记的NSF的流体动力学分析检测到NSF中的构象差异，其中ATP结合的构象比ADP结合的形式显得更紧凑。这表明NSF经历了显着的构象变化，因为它通过其ATP水解循环的进展。通过对这些数据的分析，我们提出了NSF使用NSF-N和NSF-D1分解SNAP-SNARE复合物的顺序机制。我们还说明了如何分析离心可用于研究其他AAA+蛋白质。
The N-ethylmaleimide-Sensitive Factor (NSF) was one of the initial members of the ATPases Associated with various cellular Activities Plus (AAA+) family. In this review, we discuss what is known about the mechanism of NSF action and how that relates to the mechanisms of other AAA+ proteins. Like other family members, NSF binds to a protein complex (i.e., SNAP-SNARE complex) and utilizes ATP hydrolysis to affect the conformations of that complex. SNAP-SNARE complex disassembly is essential for SNARE recycling and sustained membrane trafficking. NSF is a homo-hexamer; each protomer is composed of an N-terminal domain, NSF-N, and two adjacent AAA-domains, NSF-D1 and NSF-D2. Mutagenesis analysis has established specific roles for many of the structural elements of NSF-D1, the catalytic ATPase domain, and NSF-N, the SNAP-SNARE binding domain. Hydrodynamic analysis of NSF, labeled with (Ni2+-NTA)2-Cy3, detected conformational differences in NSF, in which the ATP-bound conformation appears more compact than the ADP-bound form. This indicates that NSF undergoes significant conformational changes as it progresses through its ATP-hydrolysis cycle. Incorporating these data, we propose a sequential mechanism by which NSF uses NSF-N and NSF-D1 to disassemble SNAP-SNARE complexes. We also illustrate how analytical centrifugation might be used to study other AAA+ proteins.
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影响因子：
16
作者：
Augustin, Steffen;Gerdes, Florian;Lee, Sukyeong;Tsai, Francis T. F.;Langer, Thomas;Tatsuta, Takashi
通讯作者：
Tatsuta, Takashi
影响因子：
3.9
作者：
Bose, Daniel;Joly, Nicolas;Zhang, Xiaodong
通讯作者：
Zhang, Xiaodong
DOI：
10.1038/nsb972
发表时间：
2003-10-01
期刊：
NATURE STRUCTURAL BIOLOGY
影响因子：
--
作者：
DeLaBarre, B;Brunger, AT
通讯作者：
Brunger, AT
影响因子：
2.9
作者：
Bitto, Eduard;Bingman, Craig A.;Phillips, George N., Jr.
通讯作者：
Phillips, George N., Jr.
DOI：
10.1073/pnas.92.15.7095
发表时间：
1995-07-18
影响因子：
11.1
作者：
BOULIANNE, GL;TRIMBLE, WS
通讯作者：
TRIMBLE, WS