The potential protective effect of immunization with outer-membrane protein I from Neisseria gonorrhoeae.
The potential protective effect of immunization with outer-membrane protein I from Neisseria gonorrhoeae.
复制标题
淋病奈瑟菌外膜蛋白 I 免疫的潜在保护作用。
DOI：
10.1099/00221287-135-8-2269
复制
发表时间：
1989
期刊：
影响因子：
--
通讯作者：
M. Virji
中科院分区：
文献类型：
--
作者：
J. Heckels;J. Fletcher;M. Virji
淋病奈瑟菌外膜免疫兔可产生针对外膜蛋白PI和PIII的抗体。针对PIII的抗体在Western blots上与所有测试的菌株反应相同，但针对PI的抗体仅与同源菌株反应。当纯化的PIB用于免疫时，免疫反应完全不同：获得的血清既可与同源和异源PIB型反应，也可与表达PIA的菌株反应。序列切割PIB产生的肽的Western blotting显示，抗om血清识别的抗原决定因子主要位于PIB的中央表面暴露区域，保护性抗PIB单克隆抗体SM24识别的表位也是如此。相反，纯化PI免疫产生的抗体与PIB n端部分的抗原决定因子反应。尽管如此，由于抗pi血清对表达PIA和pib的菌株都是阴性的，并且具有很强的杀菌作用，因此这些决定因素可以对天然蛋白进行免疫攻击。
Immunization of rabbits with outer membranes (OM) of Neisseria gonorrhoeae produced antibodies directed against outer-membrane proteins PI and PIII. The antibodies directed against PIII reacted equally well on Western blots with all strains tested, but antibodies directed against PI reacted only with the homologous strain. When purified PIB was used for immunization the immune response was quite different: the sera obtained reacted with both homologous and heterologous PIB types and also reacted with strains expressing PIA. Western blotting of peptides produced by sequential cleavage of PIB revealed that the antigenic determinants recognized by anti-OM sera were predominantly located in the central surface-exposed region of PIB, as is the epitope recognized by the protective anti-PIB monoclonal antibody SM24. In contrast antibodies produced by immunization with purified PI reacted with antigenic determinants in the N-terminal portion of PIB. Nevertheless these determinants are accessible to immune attack on the native protein since the anti-PI sera were opsonic and were strongly bactericidal for both PIA- and PIB-expressing strains.
使用淋球菌外膜蛋白 I 的单克隆抗体对淋病奈瑟菌进行血清学分类。
DOI：
10.1093/infdis/150.1.44
发表时间：
1984
期刊：
The Journal of infectious diseases
影响因子：
--
作者：
Knapp,JS;Tam,MR;Nowinski,RC;Holmes,KK;Sandström,EG
通讯作者：
Sandström,EG