Exchange-linked dissolution agents in dissolution-DNP (13)C metabolic imaging.
Exchange-linked dissolution agents in dissolution-DNP (13)C metabolic imaging.
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DOI：
10.1002/mrm.24544
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发表时间：
2013-10
影响因子：
3.3
通讯作者：
Mayer, Dirk
中科院分区：
文献类型：
--
作者：
Hurd, Ralph E.;Spielman, Daniel;Josan, Sonal;Yen, Yi-Fen;Pfefferbaum, Adolf;Mayer, Dirk
研究了在超极化代谢成像中使用未标记的交换连接溶出剂（ELDA），以检查相对于NADH可用性的池大小限制和饱和度。获得三维动态代谢图像，并在注射超极化[1- 13 C]丙酮酸盐（在溶出缓冲液中加入或不加入未标记的乳酸钠）后进行比较。根据表观速率常数和[1- 13 C]乳酸信噪比进行比较。获得了不同推注组成的范围探索数据。同位素交换也在相反方向上探测，注射推注超极化[1- 13 C]乳酸盐后，在溶解缓冲液中有和没有未标记的丙酮酸钠。在溶出缓冲液中加入未标记的乳酸钠后，肝脏、肾脏和血管相关区域均显示[1- 13 C]乳酸信号增加。注射溶出缓冲液中的超极化[1- 13 C]乳酸盐和未标记丙酮酸钠，提供了肾脏和血管相关区域的交换速率常数Klp。这些结果与高水平的13 C交换一致，并且与受体内稳态池大小限制的标记率一致。
The use of unlabeled exchange-linked dissolution agents (ELDA) in hyperpolarized metabolic imaging was studied to examine pool size limits and saturation relative to the availability of NADH. Three-dimensional dynamic metabolic images were obtained, and compared following injection of a bolus of hyperpolarized [1-13C]pyruvate, prepared with and without unlabeled sodium lactate in the dissolution buffer. Comparisons were made on the basis of apparent rate constants and [1-13C]lactate signal-to-noise ratio. Range finding data were obtained for different bolus compositions. Isotope exchange was also probed in the reverse direction, following injection of a bolus of hyperpolarized [1-13C]lactate, with and without unlabeled sodium pyruvate in the dissolution buffer. Liver, kidney and vascular regions of interest all showed an increase in [1-13C]lactate signal with addition of unlabeled sodium lactate in the dissolution buffer. Injection of hyperpolarized [1-13C]lactate with unlabeled sodium pyruvate in the dissolution buffer, provided exchange rate constants Klp for kidney and vascular regions of interest. These results are consistent with a high level of 13C-exchange, and with labeling rates that are limited by steady-state pool sizes in vivo.
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影响因子：
3.3
作者：
Spielman, Daniel M.;Mayer, Dirk;Pfefferbaum, Adolf
通讯作者：
Pfefferbaum, Adolf
影响因子：
82.9
作者：
Day, Sam E.;Kettunen, Mikko I.;Brindle, Kevin M.
通讯作者：
Brindle, Kevin M.
影响因子：
4.8
作者：
Witney, Timothy H.;Kettunen, Mikko I.;Brindle, Kevin M.
通讯作者：
Brindle, Kevin M.
影响因子：
2.9
作者：
Xu, Tao;Mayer, Dirk;Spielman, Daniel
通讯作者：
Spielman, Daniel
影响因子：
11.2
作者：
Albers MJ;Bok R;Chen AP;Cunningham CH;Zierhut ML;Zhang VY;Kohler SJ;Tropp J;Hurd RE;Yen YF;Nelson SJ;Vigneron DB;Kurhanewicz J
通讯作者：
Kurhanewicz J