Histone modifications facilitate the coexpression of bidirectional promoters in rice.
Histone modifications facilitate the coexpression of bidirectional promoters in rice.
复制标题
组蛋白修饰促进双向启动子在水稻中的共表达
DOI：
10.1186/s12864-016-3125-0
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发表时间：
2016-09-30
期刊：
影响因子：
4.4
通讯作者：
Zhang W
中科院分区：
文献类型：
--
作者：
Fang Y;Wang L;Wang X;You Q;Pan X;Xiao J;Wang XE;Wu Y;Su Z;Zhang W
背景双向基因对在真核生物基因组中含量非常丰富，且大多是共调控的。双向启动子的结构特征已经在酵母、人类和植物中得到了很好的研究。然而，导致BDPs共表达的潜在机制仍未得到充分研究，尤其是在植物中。水稻BDPs中存在一些独特的染色质特征，但在单向启动子(UDP)中缺失，包括高表达的活性组蛋白标记、典型的核小体和低表达的H3K27me3。根据Kolmogorov-Smirnov检验，H3K4ac、H4K12ac、H4K16ac、H3K4me2和H3K36me3在I型BDP中确实过表达，而在另外两个BDP中不表达。结论我们的分析表明，活性标记(H3K4ac、H4K12ac、H4K16ac、H3K4me3、H3K9ac和H3K27ac)可能与抑制标记(H3K27me3和H3K9me1/3)协同作用，构建独特的染色质结构，有利于双向基因对的协同调节。因此，我们的发现有助于加深对调控双向基因对的独特表观遗传机制的理解，并可能改善对作物生物工程基因对的操纵。
BackgroundBidirectional gene pairs are highly abundant and mostly co-regulated in eukaryotic genomes. The structural features of bidirectional promoters (BDPs) have been well studied in yeast, humans and plants. However, the underlying mechanisms responsible for the coexpression of BDPs remain understudied, especially in plants.ResultsHere, we characterized chromatin features associated with rice BDPs. Several unique chromatin features were present in rice BDPs but were missing from unidirectional promoters (UDPs), including overrepresented active histone marks, canonical nucleosomes and underrepresented H3K27me3. In particular, overrepresented active marks (H3K4ac, H4K12ac, H4K16ac, H3K4me2 and H3K36me3) were truly overrepresented in type I BDPs but not in the other two BDPs, based on a Kolmogorov-Smirnov test.ConclusionsOur analyses indicate that active marks (H3K4ac, H4K12ac, H4K16ac, H3K4me3, H3K9ac and H3K27ac) may coordinate with repressive marks (H3K27me3 and H3K9me1/3) to build a unique chromatin structure that favors the coregulation of bidirectional gene pairs. Thus, our findings help to enhance the understanding of unique epigenetic mechanisms that regulate bidirectional gene pairs and may improve the manipulation of gene pairs for crop bioengineering.
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影响因子：
2.1
作者：
Guimarães-Dias F;Neves-Borges AC;Viana AA;Mesquita RO;Romano E;de Fátima Grossi-de-Sá M;Nepomuceno AL;Loureiro ME;Alves-Ferreira M
通讯作者：
Alves-Ferreira M
影响因子：
7.4
作者：
Fincher, Justin A.;Vera, Daniel L.;Bass, Hank W.
通讯作者：
Bass, Hank W.
影响因子：
64.5
作者：
Guenther, Matthew G.;Levine, Stuart S.;Young, Richard A.
通讯作者：
Young, Richard A.
人类和小鼠基因组中的复杂基因座。
影响因子：
4.5
作者：
Engstrom, Par G.;Suzuki, Harukazu;Ninomiya, Noriko;Akalin, Altuna;Sessa, Luca;Lavorgna, Giovanni;Brozzi, Alessandro;Luzi, Lucilla;Tan, Sin Lam;Yang, Liang;Kunarso, Galih;ng, Edwin Lian-Cho Ng;Batalov, Serge;Wahlestedt, Claes;Kai, Chikatoshi;Kawai, Jun;Carninci, Piero;Hayashizaki, Yoshihide;Wells, Christine;Bajic, Vladimir B.;Orlando, Valerio;Reid, James F.;Lenhard, Boris;Lipovich, Leonard
通讯作者：
Lipovich, Leonard
DOI：
10.1111/tpj.12620
发表时间：
2014-10
期刊：
The Plant journal : for cell and molecular biology
影响因子：
--
作者：
Benn G;Wang CQ;Hicks DR;Stein J;Guthrie C;Dehesh K
通讯作者：
Dehesh K