Differential Expression of Six Rnase2 and Three Rnase3 Paralogs Identified in Blunt Snout Bream in Response to Aeromonas hydrophila Infection.
Differential Expression of Six Rnase2 and Three Rnase3 Paralogs Identified in Blunt Snout Bream in Response to Aeromonas hydrophila Infection.
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DOI：
10.3390/genes9020095
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发表时间：
2018-02-14
期刊：
影响因子：
3.5
通讯作者：
Wang W
中科院分区：
文献类型：
--
作者：
Geng R;Liu H;Wang W
核糖核酸酶（Rnases）2和Rnase3属于核糖核酸酶A （RnaseA）超家族。除了在分子进化和功能生物学研究中的作用外，这些基因也在哺乳动物防御病原体感染的背景下进行了研究。然而，这两个基因在钝口鲷（Megalobrama amblycepphala）中的表达模式、结构和对细菌感染的反应尚不清楚。在这项研究中，我们在M. amblycephala基因组中鉴定了Rnase2(6)和Rnase3(3)的多个拷贝。这9个基因都具有RnaseA超家族的典型特征。Rnase2b、Rnase2c、Rnase2e和Rnase3a在受精后120和140 h （hpf）弱表达，表明Rnase2和rnasee3分别只表达了3个拷贝和1个拷贝。有趣的是，感染嗜水气单胞菌后，只有Rnase2e在双头单胞菌的肾脏中表达上调，而Rnase3a在肝脏、肠道和血液中表达显著上调。我们认为Rnase3的同源物比Rnase2更容易感染嗜水单胞菌。这些结果表明，不同的Rnase2和Rnase3同源物可能在双头棘球绦虫对细菌感染的先天免疫应答中起作用。
Ribonucleases (Rnases)2 and Rnase3 belong to the ribonuclease A (RnaseA) superfamily. Apart from their role in molecular evolutionary and functional biological studies, these genes have also been studied in the context of defense against pathogen infection in mammals. However, expression patterns, structures and response to bacterial infection of the two genes in blunt snout bream (Megalobrama amblycephala) remain unknown. In this study, we identified multiple copies of Rnase2 (six) and Rnase3 (three) in the M. amblycephala genome. The nine genes all possess characteristics typical of the RnaseA superfamily. No expression was detected in the early developmental stages, while a weak expression was observed at 120 and 140 h post-fertilization (hpf) for Rnase2b, Rnase2c, Rnase2e and Rnase3a, suggesting that only three copies of Rnase2 and one of Rnase3 are expressed. Interestingly, only Rnase2e was up-regulated in the kidney of M. amblycephala after Aeromonas hydrophila infection, while Rnase3a was significantly up-regulated in liver, gut and blood after the infection. We conclude that the paralogs of Rnase3 are more susceptible to A. hydrophila infection than Rnase2. These results indicate that different Rnase2 and Rnase3 paralogs suggest a role in the innate immune response of M. amblycephala to bacterial infection.
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影响因子：
5.6
作者：
Kazakou, Konstantina;Holloway, Daniel E.;Prior, Stephen H.;Subramanian, Vasanta;Acharya, K. Ravi
通讯作者：
Acharya, K. Ravi
DOI：
10.1016/s0006-291x(86)80310-2
发表时间：
1986-09-30
影响因子：
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通讯作者：
Pham BN
影响因子：
10.7
作者：
Cho, Soochin;Zhang, Jianzhi
通讯作者：
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