Structure and inherent properties of the bacteriophage lambda head shell. VII. Molecular design of the form-determining major capsid protein.
Structure and inherent properties of the bacteriophage lambda head shell. VII. Molecular design of the form-determining major capsid protein.
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噬菌体 lambda 头壳的结构和固有特性。
DOI：
10.1016/s0022-2836(05)80211-6
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发表时间：
1983
影响因子：
5.6
通讯作者：
H. Kobayashi
中科院分区：
文献类型：
--
作者：
I. Katsura;H. Kobayashi
λ噬菌体主要衣壳蛋白（gpE）的一些突变可以改变头壳的大小和形状或阻断头成熟的途径。以往对这类突变体分类的研究表明，gpE分子上至少有5个功能位点。在这项研究中，我们通过DNA测序来确定氨基酸交换，以阐明形状决定多功能蛋白gpE的分子设计。此外，我们通过二维凝胶电泳表征了突变的gpE分子，并研究了琥珀色突变体在43个氨基酸残基上的抑制模式。这些突变映射在22个碱基的19个氨基酸残基上，分别位于gpE 341个氨基酸残基中的40 ~ 91、222 ~ 246和284 ~ 324三个区域。这些区域似乎对gpE的活性很重要，因为这些区域的琥珀色突变被较少种类的抑制子抑制，而非这些区域。具有不同表型的突变彼此不分离，而具有相同表型的一些突变在初级结构中分离得很远。这表明这些功能位点是在祖先gpE多肽链的折叠模式建立之后在进化过程中形成的。许多突变位于丝氨酸、甘氨酸和脯氨酸残基的预测β-转位。
Some mutations in the major capsid protein (gpE) ofλphage can alter the size and shape of the head shell or block the pathway of head maturation. Previous studies on the classification of such mutants showed that there are at least five functional sites on the gpE molecule. In this study, we determined the amino acid exchanges by DNA sequencing to elucidate the molecular design of the form-determining multifunctional protein gpE. In addition, we characterized the mutated gpE molecules by two-dimensional gel electrophoresis and studied suppression patterns of amber mutants at 43 amino acid residues.Those mutations map at 19 amino acid residues at 22 bases, which are located in three regions, 40 to 91, 222 to 246, and 284 to 324 of the 341 amino acid residues of gpE. These regions seem to be important in the activity of gpE, since amber mutations in these regions are suppressed on the average by less species of suppressors than those outside these regions. The mutations having different phenotypes are not segregated from each other, while some mutations having the same phenotype are separated far apart in the primary structure. This suggests that the functional sites were formed during evolution after the folding pattern of the ancestral gpE polypeptide chain had been established. Many of the mutations are located at serine, glycine and proline residues in predictedβ-turns.
影响因子：
3.4
作者：
S. Harrison
通讯作者：
S. Harrison
λ噬菌体的头尾连接器。
影响因子：
5.6
作者：
Tsui,L;Hendrix,RW
通讯作者：
Hendrix,RW
南方豆花叶病毒的亚基相互作用。
影响因子：
5.6
作者：
Rossmann,MG;Abad-Zapatero,C;Hermodson,MA;Erickson,JW
通讯作者：
Erickson,JW