Dormant and after-Ripened Arabidopsis thaliana Seeds are Distinguished by Early Transcriptional Differences in the Imbibed State.
Dormant and after-Ripened Arabidopsis thaliana Seeds are Distinguished by Early Transcriptional Differences in the Imbibed State.
复制标题
DOI：
10.3389/fpls.2016.01323
复制
发表时间：
2016
影响因子：
5.6
通讯作者：
Bentsink L
中科院分区：
文献类型：
--
作者：
Dekkers BJ;Pearce SP;van Bolderen-Veldkamp RP;Holdsworth MJ;Bentsink L
种子休眠是一种基因控制的阻止，阻止吸水种子在有利条件下发芽。需要一段时间的干燥储存（后熟）或克服一定的环境条件。休眠是一个重要的种子性状，它在选择压力下控制种子发芽的季节时间。休眠和非休眠（后熟）种子的特征是大量差异表达的基因。然而，关于休眠和非休眠种子再水化早期阶段的时间和空间转录变化的信息很少。我们对模式植物拟南芥的种子进行了全基因组转录组分析，以研究干燥种子在再水化后的转录变化。我们分析了播种后 24 小时内 Cvi 种质的休眠和后熟种子在四个时间点和两个种子隔室（胚胎和周围单细胞层胚乳）的基因表达。这项工作提供了休眠和非休眠种子基因表达变化的全局视图，包括时间和空间细节，并且这些可以通过网络可访问工具（http://www.wageningenseedlab.nl/resources）可视化。在再水化时，休眠和非休眠种子中的大部分转录本发生相似的变化，然而，在吸胀开始后不久，转录本丰度的第一个差异变得可见，这表明后熟引起的变化在再水化时被检测到并迅速做出反应。我们确定了几种基因表达谱，它们有助于休眠和非休眠样品之间基因表达的差异。在与胁迫相关的基因本体论类别中，休眠种子胚乳中表达增强的基因被过度代表，这表明胚乳对生物和非生物胁迫具有保护作用，以支持休眠种子在其环境中的持久存在。
Seed dormancy is a genetically controlled block preventing the germination of imbibed seeds in favorable conditions. It requires a period of dry storage (after-ripening) or certain environmental conditions to be overcome. Dormancy is an important seed trait, which is under selective pressure, to control the seasonal timing of seed germination. Dormant and non-dormant (after-ripened) seeds are characterized by large sets of differentially expressed genes. However, little information is available concerning the temporal and spatial transcriptional changes during early stages of rehydration in dormant and non-dormant seeds. We employed genome-wide transcriptome analysis on seeds of the model plant Arabidopsis thaliana to investigate transcriptional changes in dry seeds upon rehydration. We analyzed gene expression of dormant and after-ripened seeds of the Cvi accession over four time points and two seed compartments (the embryo and surrounding single cell layer endosperm), during the first 24 h after sowing. This work provides a global view of gene expression changes in dormant and non-dormant seeds with temporal and spatial detail, and these may be visualized via a web accessible tool (http://www.wageningenseedlab.nl/resources). A large proportion of transcripts change similarly in both dormant and non-dormant seeds upon rehydration, however, the first differences in transcript abundances become visible shortly after the initiation of imbibition, indicating that changes induced by after-ripening are detected and responded to rapidly upon rehydration. We identified several gene expression profiles which contribute to differential gene expression between dormant and non-dormant samples. Genes with enhanced expression in the endosperm of dormant seeds were overrepresented for stress-related Gene Ontology categories, suggesting a protective role for the endosperm against biotic and abiotic stress to support persistence of the dormant seed in its environment.
登录
查看更多内容
DOI：
10.1073/pnas.1000410107
发表时间：
2010-03-02
影响因子：
11.1
作者：
Bentsink, Leonie;Hanson, Johannes;Koornneef, Maarten
通讯作者：
Koornneef, Maarten
DOI：
10.1199/tab.0119
发表时间：
2008-01-01
期刊：
The arabidopsis book
影响因子：
--
作者：
Bentsink, Leonie;Koornneef, Maarten
通讯作者：
Koornneef, Maarten
影响因子：
7.2
作者：
Carrera, Esther;Holman, Tara;Holdsworth, Michael J.
通讯作者：
Holdsworth, Michael J.
影响因子：
4.5
作者：
De Giorgi J;Piskurewicz U;Loubery S;Utz-Pugin A;Bailly C;Mène-Saffrané L;Lopez-Molina L
通讯作者：
Lopez-Molina L
影响因子：
7.2
作者：
Finch-Savage, William E.;Cadman, Cassandra S. C.;Hilhorst, Henk W. M.
通讯作者：
Hilhorst, Henk W. M.