Photosensitizer-conjugated gold nanorods for enzyme-activatable fluorescence imaging and photodynamic therapy.
Photosensitizer-conjugated gold nanorods for enzyme-activatable fluorescence imaging and photodynamic therapy.
复制标题
作者：
Jang B;Choi Y
我们报道了光敏剂偶联金纳米棒（mmp2b -GNR）的发展，其中光敏剂通过蛋白酶可切割肽连接剂偶联到金纳米棒（GNR）的表面。我们假设共轭光敏剂的荧光和光毒性在其天然状态下会被抑制，只有在被靶蛋白酶基质金属蛋白酶2 （MMP2）切割后才会被激活。荧光和单重态产氧（SOG）的定量分析表明，与游离焦磷化合物-a相比，MMP2P-GNR共轭物的荧光强度为0.4%±0.01%，SOG效率为0.89%±1.04%。通过使用过表达MMP2的HT1080细胞和缺乏MMP2的BT20细胞的体外细胞研究，我们观察到这些细胞系中MMP2的存在或不存在介导了荧光和SOG。这种新型的可活化光敏系统可用于蛋白酶介导的荧光成像和随后的各种癌症的光动力治疗。
We report on the development of photosensitizer-conjugated gold nanorods (MMP2P-GNR) in which photosensitizers were conjugated onto the surface of gold nanorods (GNR) via a protease-cleavable peptide linker. We hypothesized that fluorescence and phototoxicity of the conjugated photosensitizers would be suppressed in their native state, becoming activated only after cleavage by the target protease matrix metalloprotease-2 (MMP2). Quantitative analysis of the fluorescence and singlet oxygen generation (SOG) demonstrated that the MMP2P-GNR conjugate emitted fluorescence intensity corresponding to 0.4% ± 0.01% and an SOG efficiency of 0.89% ± 1.04% compared to free pyropheophorbide-a. From the in vitro cell studies using HT1080 cells that overexpress MMP2 and BT20 cells that lack MMP2, we observed that fluorescence and SOG was mediated by the presence or absence of MMP2 in these cell lines. This novel activatable photosensitizing system may be useful for protease-mediated fluorescence imaging and subsequent photodynamic therapy for various cancers.
登录
查看更多内容
影响因子：
17.1
作者：
Jang, Boseung;Park, Jin-Young;Choi, Yongdoo
通讯作者：
Choi, Yongdoo
影响因子：
3.4
作者：
McCarthy, Jason R.;Weissleder, Ralph
通讯作者：
Weissleder, Ralph
DOI：
10.1111/j.1749-6632.1999.tb07687.x
发表时间：
1999-01-01
期刊：
INHIBITION OF MATRIX METALLOPROTEINASES: THERAPEUTIC APPLICATIONS
影响因子：
--
作者：
Zucker, S;Hymowitz, M;Montana, S
通讯作者：
Montana, S
影响因子：
82.9
作者：
Bremer, C;Tung, CH;Weissleder, R
通讯作者：
Weissleder, R
影响因子：
16.6
作者：
Park, So Young;Baik, Hye Jung;Lee, Eun Seong
通讯作者：
Lee, Eun Seong