Growth phase-dependent expression of the Pseudomonas putida KT2440 transcriptional machinery analysed with a genome-wide DNA microarray.
Growth phase-dependent expression of the Pseudomonas putida KT2440 transcriptional machinery analysed with a genome-wide DNA microarray.
复制标题
使用全基因组 DNA 微阵列分析恶臭假单胞菌 KT2440 转录机制的生长阶段依赖性表达。
DOI：
10.1111/j.1462-2920.2005.00890.x
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发表时间：
2006
影响因子：
5.1
通讯作者：
F. Rojo
中科院分区：
文献类型：
--
作者：
Luís Yuste;A. Hervás;I. Canosa;R. Tobes;José I. Jiménez;Juan Nogales;Manuel M. Pérez;E. Santero;E. Díaz;J. Ramos;V. de Lorenzo;F. Rojo
细菌转录网络建立在调节器的层次结构上，在其之上是RNA聚合酶的组分（特别是sigma因子）和全局控制元件，其发挥关键作用。我们设计了一个全基因组的恶臭假单胞菌KT 2440的DNA微阵列。结合实时逆转录聚合酶链反应（RT-PCR），我们用它来分析编码RNA聚合酶亚基的基因的表达模式（核心酶和24 σ因子），以及参与全球调节的各种蛋白质（Crc、Lrp、Fur、Anr、Fis、CsrA、IHF、HupA、HupB、HupN、BipA和几种MvaT样蛋白），在从丰富培养基中的指数生长到进入稳定期所带来的饥饿和胁迫的转变期间。RNA聚合酶核心基因和营养期sigma因子基因的表达在稳定期下降，而sigma（S）基因的表达增加。sigma（N）、sigma（H）、FliA和19种细胞质外功能（ECF）样sigma因子的数据表明，它们的mRNA水平在进入稳定期后变化很小。稳定期Crc、BipA、Fis、HupB、HupN和MvaT样蛋白PP 3693表达下降，而HupA和MvaT样蛋白PP 3765表达明显增加。IHF的表达指示转录后控制。这些结果提供了通过恶臭假单胞菌中的指数稳态相移表达转录机器的第一个全球性研究。
Bacterial transcriptional networks are built on a hierarchy of regulators, on top of which lie the components of the RNA polymerase (in particular the sigma factors) and the global control elements, which play a pivotal role. We have designed a genome-wide oligonucleotide-based DNA microarray for Pseudomonas putida KT2440. In combination with real-time reverse transcription polymerase chain reaction (RT-PCR), we have used it to analyse the expression pattern of the genes encoding the RNA polymerase subunits (the core enzyme and the 24 sigma factors), and various proteins involved in global regulation (Crc, Lrp, Fur, Anr, Fis, CsrA, IHF, HupA, HupB, HupN, BipA and several MvaT-like proteins), during the shift from exponential growth in rich medium into starvation and stress brought about by the entry into stationary phase. Expression of the genes encoding the RNA polymerase core and the vegetative sigma factor decreased in stationary phase, while that of sigma(S) increased. Data obtained for sigma(N), sigma(H), FliA and for the 19 extracytoplasmic function (ECF)-like sigma factors suggested that their mRNA levels change little upon entry into stationary phase. Expression of Crc, BipA, Fis, HupB, HupN and the MvaT-like protein PP3693 decreased in stationary phase, while that of HupA and the MvaT-like protein PP3765 increased significantly. Expression of IHF was indicative of post-transcriptional control. These results provide the first global study of the expression of the transcriptional machinery through the exponential stationary-phase shift in P. putida.
DOI：
10.1073/pnas.220414297
发表时间：
2000-10-24
影响因子：
11.1
作者：
Khodursky, AB;Peter, BJ;Yanofsky, C
通讯作者：
Yanofsky, C
DOI：
10.1073/pnas.98.3.892
发表时间：
2001-01-30
影响因子：
11.1
作者：
Minakhin, L;Bhagat, S;Severinov, K
通讯作者：
Severinov, K