Optimisation of experimental conditions for synthesis of high affinity MIP nanoparticles
Optimisation of experimental conditions for synthesis of high affinity MIP nanoparticles
复制标题
高亲和力MIP纳米粒子合成实验条件优化
DOI：
10.1016/j.eurpolymj.2012.09.022
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发表时间：
2013
影响因子：
6
通讯作者：
S. Piletsky
中科院分区：
文献类型：
--
作者：
S. Subrahmanyam;A. Guerreiro;A. Poma;E. Moczko;E. Piletska;S. Piletsky
在链终止剂存在下，在高单体和模板浓度下，通过iniferter聚合合成分子印迹纳米颗粒。优化引发剂、链终止剂和UV照射时间的量，以实现离散纳米颗粒的最大产率，而不引起聚合混合物的大凝胶化。然后通过凝胶渗透从未反应的单体中纯化所得颗粒，并通过尺寸排阻色谱法、动态光散射和电子显微镜进行表征。通过固定化模板上的表面等离子体共振验证对靶标的亲和力。使用这种方法获得的纳米粒子的产率为14%，与使用高单体浓度和UV引发的类似方法相比，显着增加。
Molecularly imprinted nanoparticles were synthesised via iniferter polymerisation in the presence of a chain terminator agent and at high monomer and template concentration. The amount of initiator, chain terminator agent and UV irradiation time were optimised in order to achieve maximum yield of discrete nanoparticles without causing macrogelation of the polymerisation mixture. Resultant particles were then purified from unreacted monomers via gel permeation and characterised by size-exclusion chromatography, dynamic light scattering and electron microscopy. The affinity towards the target was verified via surface plasmon resonance on immobilised templates. The yield of nanoparticles obtained using this approach was 14%, a significant increase when compared with similar methods using high monomer concentration and UV initiation.
影响因子：
15
作者：
Hoshino, Yu;Koide, Hiroyuki;Urakami, Takeo;Kanazawa, Hiroaki;Kodama, Takashi;Oku, Naoto;Shea, Kenneth J.
通讯作者：
Shea, Kenneth J.