Recognition, neutralization, and clearance of target peptides in the bloodstream of living mice by molecularly imprinted polymer nanoparticles: a plastic antibody.
Recognition, neutralization, and clearance of target peptides in the bloodstream of living mice by molecularly imprinted polymer nanoparticles: a plastic antibody.
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DOI：
10.1021/ja102148f
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发表时间：
2010-05-19
影响因子：
15
通讯作者：
Shea, Kenneth J.
中科院分区：
文献类型：
--
作者：
Hoshino, Yu;Koide, Hiroyuki;Urakami, Takeo;Kanazawa, Hiroaki;Kodama, Takashi;Oku, Naoto;Shea, Kenneth J.
我们报告说，简单的合成有机聚合物纳米颗粒（NPs）可以捕获和清除活小鼠血流中的目标肽毒素。蛋白质大小的聚合物纳米粒子，具有与天然抗体的结合亲和力和选择性相媲美，通过将功能单体优化策略与分子印迹纳米粒子合成相结合制备。由于结合和去除蜂毒素的纳米粒子在体内，死亡率和外周中毒症状的蜂毒素显着减少。聚合物纳米颗粒或“塑料抗体”的体内成像确定了纳米颗粒加速肽从血液中的清除，其中肽在肝脏中积累。再加上它们的生物相容性和无毒特性，塑料抗体提供了在体内中和广泛的生物大分子的潜力。
We report that simple, synthetic organic polymer nanoparticles (NPs) can capture and clear a target peptide toxin in the bloodstream of living mice. The protein-size polymer nanoparticles, with a binding affinity and selectivity comparable to natural antibodies, were prepared by combining a functional monomer optimization strategy with molecular imprinting nanoparticle synthesis. As a result of binding and removal of melittin by NPs in vivo, mortality and peripheral toxic symptoms of melittin were significantly diminished. In vivo imaging of the polymer nanoparticles or “plastic antibodies” establishes the NPs accelerate clearance of the peptide from blood where they accumulate in the liver. Coupled with their biocompatibility and nontoxic characteristics, plastic antibodies offer potential for neutralizing a wide range of biomacromolecules in vivo.
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影响因子：
15
作者：
Verma, A;Nakade, H;Rotello, VM
通讯作者：
Rotello, VM
影响因子：
56.9
作者：
HABERMAN.E
通讯作者：
HABERMAN.E
影响因子：
3.9
作者：
Aubin-Tam, ME;Hamad-Schifferli, K
通讯作者：
Hamad-Schifferli, K
影响因子：
13.3
作者：
Hoshino, Yu;Urakami, Takeo;Kodama, Takashi;Koide, Hiroyuki;Oku, Naoto;Okahata, Yoshio;Shea, Kenneth J.
通讯作者：
Shea, Kenneth J.
影响因子：
7.4
作者：
Haupt, K
通讯作者：
Haupt, K