miR1432-OsACOT (Acyl-CoA thioesterase) module determines grain yield via enhancing grain filling rate in rice.
miR1432-OsACOT (Acyl-CoA thioesterase) module determines grain yield via enhancing grain filling rate in rice.
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miR1432-OsACOT（酰基-CoA硫酯酶）模块通过提高水稻籽粒灌浆率来确定籽粒产量
DOI：
10.1111/pbi.13009
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发表时间：
2019-04
影响因子：
13.8
通讯作者：
Zhao QZ
中科院分区：
文献类型：
--
作者：
Zhao YF;Peng T;Sun HZ;Teotia S;Wen HL;Du YX;Zhang J;Li JZ;Tang GL;Xue HW;Zhao QZ
水稻籽粒灌浆速率对籽粒生产力和养分积累有很大贡献。microRNAs（miRNAs）是植物生长发育和生理的重要调控因子，是实现作物籽粒大小和产量工程化的重要靶点。然而，目前关于miRNA对水稻灌浆和产量调控的研究还很少。在这里，我们表明，抑制水稻miR 1432（STTM 1432）的表达显着提高粒重，通过提高灌浆速率，并导致在田间试验中的总籽粒产量增加高达17.14%。分子分析鉴定了水稻酰基辅酶A硫酯酶（OsACOT），其在其他物种中与ACOT 13保守，作为miR 1432通过切割的主要靶标。此外，过表达miR 1432抗性形式的OsACOT（OXmACOT）类似于STTM 1432植物，即通过提高籽粒灌浆速率而使粒重大幅度增加高达46.69%。进一步的研究表明OsACOT参与了中链脂肪酸的生物合成。此外，对miR 1432表达改变的转基因植物的基于RNA-seq的转录组学分析表明，miR 1432调控网络的下游基因参与脂肪酸代谢和植物激素的生物合成，并且还与OsACOT共表达基因的富集分析重叠，这与STTM 1432和OXmACOT植物中生长素和脱落酸水平的增加一致。总体而言，miR 1432-OsACOT模块在水稻籽粒灌浆中发挥重要作用，说明其在作物工程产量提高中的能力。
Rice grain filling rate contributes largely to grain productivity and accumulation of nutrients. MicroRNAs (miRNAs) are key regulators of development and physiology in plants and become a novel key target for engineering grain size and crop yield. However, there is little studies, so far, showing the miRNA regulation of grain filling and rice yield, in consequence. Here, we show that suppressed expression of rice miR1432 (STTM1432) significantly improves grain weight by enhancing grain filling rate and leads to an increase in overall grain yield up to 17.14% in a field trial. Molecular analysis identified rice Acyl‐CoA thioesterase (OsACOT), which is conserved with ACOT13 in other species, as a major target of miR1432 by cleavage. Moreover, overexpression of miR1432‐resistant form of OsACOT (OXmACOT) resembled the STTM1432 plants, that is, a large margin of an increase in grain weight up to 46.69% through improving the grain filling rate. Further study indicated that OsACOT was involved in biosynthesis of medium‐chain fatty acids. In addition, RNA‐seq based transcriptomic analyses of transgenic plants with altered expression of miR1432 demonstrated that downstream genes of miR1432‐regulated network are involved in fatty acid metabolism and phytohormones biosynthesis and also overlap with the enrichment analysis of co‐expressed genes of OsACOT, which is consistent with the increased levels of auxin and abscisic acid in STTM1432 and OXmACOT plants. Overall, miR1432‐OsACOT module plays an important role in grain filling in rice, illustrating its capacity for engineering yield improvement in crops.
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影响因子：
5.3
作者：
Ellis, Jessica M.;Wong, William;Wolfgang, Michael J.
通讯作者：
Wolfgang, Michael J.
影响因子：
6.9
作者：
Jiang D;Chen W;Dong J;Li J;Yang F;Wu Z;Zhou H;Wang W;Zhuang C
通讯作者：
Zhuang C
DOI：
10.1111/j.2517-6161.1995.tb02031.x
发表时间：
1995-01-01
影响因子：
5.8
作者：
BENJAMINI, Y;HOCHBERG, Y
通讯作者：
HOCHBERG, Y
影响因子：
30.8
作者：
Miura, Kotaro;Ikeda, Mayuko;Ashikari, Motoyuki
通讯作者：
Ashikari, Motoyuki
植物中非常规蛋白质分泌（UPS）途径
影响因子：
7.5
作者：
Ding, Yu;Robinson, David G.;Jiang, Liwen
通讯作者：
Jiang, Liwen