Overexpression of miR164b-resistant OsNAC2 improves plant architecture and grain yield in rice.
Overexpression of miR164b-resistant OsNAC2 improves plant architecture and grain yield in rice.
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DOI：
10.1093/jxb/ery017
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发表时间：
2018-03-24
影响因子：
6.9
通讯作者：
Zhuang C
中科院分区：
文献类型：
--
作者：
Jiang D;Chen W;Dong J;Li J;Yang F;Wu Z;Zhou H;Wang W;Zhuang C
植物结构影响粮食产量。在田间条件下，过表达一种抗miRNA降解的OsNAC2基因可以增加穗数和籽粒产量，从而确定了一种新的增产策略。植物构型是水稻育种和选择的主要目标，但潜在的调控网络仍不清楚。在这里，我们过度表达了一个不能被miRNA miR164b切割的OsNAC2突变体(OERN)。OERN植株具有更好的植株结构和更长的圆锥花序，并产生更多的粮食。亲本平均主穗一次分枝数为12.2个，二次分枝数为31.5个；两个OERN系主穗平均一次分枝数分别为15.0个和15.2个，二次分枝数为41.5个和44.3个。在大规模的田间试验中，OERN植株的总粒产量(按重量计)比亲本至少多58.62%。茎、节间和叶片中也有较多的大小维管束。MiR164b基因的过表达或OsNAC2基因的下调导致主穗长度和产量下降。OERN植株的籽粒数和植株结构相关基因IPA1和DEP1显著上调。一项对3000个水稻品种的调查发现，OsNAC2的miR164b结合位点没有自然突变。OERN提高了日本晴和常用品种扬玉净3号的产量。综上所述，我们确定了通过过表达OsmiR164b抗性OsNAC2来大幅提高水稻产量和改善植株结构的有效新策略。
Plant architecture affects grain yield. Overexpressing a version of OsNAC2 resistant to miRNA-mediated degradation increased panicle number and grain yield under field conditions, identifying a new strategy to improve yield. Plant architecture is a major target of rice (Oryza sativa) breeding and selection, but the underlying regulatory networks remain unclear. Here, we overexpressed an OsNAC2 mutant (OErN) that cannot be cleaved by the miRNA miR164b. OErN plants had better plant architecture and longer panicles, and produced more grains. The parental line averaged 12.2 primary and 31.5 secondary branches in the main panicles; two OErN lines averaged 15.0 and 15.2 primary, and 41.5 and 44.3 secondary branches. In large-scale field trials, OErN plants produced at least 58.62% more total grain (by weight) compared with the parental line. They also had more large and small vascular bundles in the stem internodes and leaves. Overexpression of miR164b or down-regulation of OsNAC2 led to decreased panicle length and grain yield in the main panicle. The OErN plants showed significant up-regulation of the grain number and plant architecture-related genes IPA1 and DEP1. A survey of >3000 rice varieties found no natural mutations in the miR164b-binding site of OsNAC2. OErN increased yield in Nipponbare and the commonly grown Yangyujing 3 cultivars. In summary, we identified an efficient new strategy to increase rice yield substantially and improve plant architecture through overexpression of OsmiR164b-resistant OsNAC2.
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影响因子：
14.9
作者：
Chen C;Ridzon DA;Broomer AJ;Zhou Z;Lee DH;Nguyen JT;Barbisin M;Xu NL;Mahuvakar VR;Andersen MR;Lao KQ;Livak KJ;Guegler KJ
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