The role of alternative oxidase in tomato fruit ripening and its regulatory interaction with ethylene.
The role of alternative oxidase in tomato fruit ripening and its regulatory interaction with ethylene.
复制标题
替代氧化酶在番茄果实成熟中的作用及其与乙烯的调控相互作用
DOI：
10.1093/jxb/ers226
复制
发表时间：
2012-09
影响因子：
6.9
通讯作者：
Lin HH
中科院分区：
文献类型：
--
作者：
Xu F;Yuan S;Zhang DW;Lv X;Lin HH
虽然交替氧化酶（AOX）已被提出在果实发育中发挥作用，但AOX在果实成熟中的功能尚不清楚。为了进一步了解AOX在番茄果实成熟中的作用，产生了转基因番茄植物35 S-AOX 1a和35 S-AOX-RNAi。番茄植株与LeAOX水平降低表现出延迟成熟;减少类胡萝卜素，呼吸，乙烯的产生;和成熟相关基因的下调。此外，AOX降低的番茄果实没有发生明显的呼吸跃变，表明AOX可能在跃变呼吸中起重要作用。相比之下，过表达LeAOX 1a的果实积累了更多的番茄红素，尽管它们表现出与野生型果实相似的成熟模式。乙烯的应用促进果实成熟和预期的乙烯产生和呼吸，包括交替途径呼吸。有趣的是，LeAOX水平降低的转基因植物在1-甲基环丙烯（1-MCP）处理后未能成熟，而这种抑制在35 S-AOX 1a果实中明显不太有效。这些结果表明，AOX参与呼吸跃变和乙烯介导的番茄果实成熟。
Although the alternative oxidase (AOX) has been proposed to play a role in fruit development, the function of AOX in fruit ripening is unclear. To gain further insight into the role of AOX in tomato fruit ripening, transgenic tomato plants 35S-AOX1a and 35S-AOX-RNAi were generated. Tomato plants with reduced LeAOX levels exhibited retarded ripening; reduced carotenoids, respiration, and ethylene production; and the down-regulation of ripening-associated genes. Moreover, no apparent respiratory climacteric occurred in the AOX-reduced tomato fruit, indicating that AOX might play an important role in climacteric respiration. In contrast, the fruit that overexpressed LeAOX1a accumulated more lycopene, though they displayed a similar pattern of ripening to wild-type fruit. Ethylene application promoted fruit ripening and anticipated ethylene production and respiration, including the alternative pathway respiration. Interestingly, the transgenic plants with reduced LeAOX levels failed to ripen after 1-methylcyclopropene (1-MCP) treatment, while such inhibition was notably less effective in 35S-AOX1a fruit. These findings indicate that AOX is involved in respiratory climacteric and ethylene-mediated fruit ripening of tomato.
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影响因子：
6.1
作者：
Dicenta, F;Martínez-Gómez, P;Berenguer, V
通讯作者：
Berenguer, V
影响因子：
3
作者：
Costa, ADT;Nantes, IL;Vercesi, AE
通讯作者：
Vercesi, AE
影响因子：
7.4
作者：
Alba, R;Cordonnier-Pratt, MM;Pratt, LH
通讯作者：
Pratt, LH
影响因子：
7.4
作者：
Ederli, Luisa;Morettini, Roberta;Pasqualini, Stefania
通讯作者：
Pasqualini, Stefania
抑制转基因植物乙烯激素合成的反义基因
影响因子：
64.8
作者：
HAMILTON, AJ;LYCETT, GW;GRIERSON, D
通讯作者：
GRIERSON, D