Polyclonal anti-Candida antibody improves phagocytosis and overall outcome in zebrafish model of disseminated candidiasis.
Polyclonal anti-Candida antibody improves phagocytosis and overall outcome in zebrafish model of disseminated candidiasis.
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DOI：
10.1016/j.dci.2016.11.017
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发表时间：
2017-03
影响因子：
2.9
通讯作者：
Wheeler RT
中科院分区：
文献类型：
--
作者：
Bergeron AC;Barker SE;Brothers KM;Prasad BC;Wheeler RT
真菌感染是全世界动植物发病和死亡的主要原因。有效的生物疗法可以补充目前的抗真菌药物，但其体内机制的理解一直受到阻碍的技术障碍，活体成像的宿主-病原体相互作用。在这里，我们通过对这些透明动物进行活体成像，将斑马鱼的真菌感染描述为了解生物抗真菌疗法作用机制的模型。我们发现，非特异性的人IgG增强体内斑马鱼吞噬细胞的吞噬作用。多克隆抗念珠菌抗体增强体内真菌的遏制并促进存活。分析表明，早期吞噬细胞遏制是一个强有力的预后指标的总生存。虽然多克隆抗念珠菌抗体可以预防疾病，但对于单个单克隆抗念珠菌抗体来说，情况并不一定如此。因此，斑马鱼似乎提供了一个有用的模型宿主，用于测试生物治疗剂是否促进体内吞噬作用并增强对念珠菌血症的保护。
Fungal infections are a major cause of animal and plant morbidity and mortality worldwide. Effective biological therapeutics could complement current antifungal drugs, but understanding of their in vivo mechanisms has been hampered by technical barriers to intravital imaging of host-pathogen interactions. Here we characterize the fungal infection of zebrafish as a model to understand the mechanism-of-action for biological antifungal therapeutics through intravital imaging of these transparent animals. We find that non-specific human IgG enhances phagocytosis by zebrafish phagocytes in vivo. Polyclonal anti-Candida antibodies enhance containment of fungi in vivo and promote survival. Analysis suggests that early phagocytic containment is a strong prognostic indicator for overall survival. Although polyclonal anti-Candida antibodies protect against disease, this is not necessarily the case for individual monoclonal anti-Candida antibodies. Thus, the zebrafish appears to provide a useful model host for testing if a biological therapeutic promotes phagocytosis in vivo and enhances protection against candidemia.
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影响因子：
11.8
作者：
Horn, David L.;Neofytos, Dionissios;Webster, Karen M.
通讯作者：
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Wheeler RT
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通讯作者：
Wheeler, Robert T.
影响因子：
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作者：
Gratacap RL;Rawls JF;Wheeler RT
通讯作者：
Wheeler RT