Calcium ATPase activity and membrane structure in clear and cataractous human lenses.
Calcium ATPase activity and membrane structure in clear and cataractous human lenses.
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透明和白内障人类晶状体中的钙 ATP 酶活性和膜结构。
DOI：
10.1076/ceyr.16.4.333.10689
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发表时间：
1997
影响因子：
2
通讯作者：
Jimenez-Asensio,J
中科院分区：
文献类型：
--
作者：
Paterson,CA;Zeng,J;Husseini,Z;Borchman,D;Delamere,NA;Garland,D;Jimenez-Asensio,J
PURPOSEMaintenance of calcium homeostasis is imperative for the clarity of the lens. Ca 2+ -ATPase is essential for the removal of cytosolic calcium, either across the plasma membrane or through intracellular organelles such as the endoplasmic reticulum. In this study, membranes prepared from clear lens epithelium were compared to membranes prepared from cataractous lens epithelium.METHODSHuman lens membranes were prepared by a protocol utilizing homogenization and centrifugation. Ca 2+ -ATPase activity was measured biochemically using Gamma- 32 P labeled ATP. Lipid order was measured using infrared and Raman spectroscopy.RESULTSCa 2+ -ATPase activity was similar in membranes prepared from cataractous lenses that were classified as nuclear subcapsular, nuclear and brunescent cataracts. Ca 2+ -ATPase activity was approximately 50% less in membranes prepared from cataractous lenses in comparison to clear lenses. Because clear lenses from Indian donors was unavailable, clear human lenses were used as a qualitative control for the measurement for Ca 2+ -ATPase activity. Lipid order was measured in lens fibers from cataractous and clear lenses from the United States donors. Lipid order increased from 55% in the hydrocarbon chains from clear lens fibers to 84% in cataractous lens fibers.CONCLUSIONSThese findings support the hypothesis that membranes are deranged in cataractous tissue, which should lead to altered levels of calcium.
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眼睛晶状体中过氧化氢选择性抑制膜 ATP 酶。
DOI：
--
发表时间：
1988
期刊：
Basic life sciences
影响因子：
--
作者：
D. Borchman;C. Paterson;N. Delamere
通讯作者：
N. Delamere
钙对晶状体ATP酶活性的影响。
影响因子：
4.4
作者：
P. M. Hamilton;N. Delamere;C. Paterson
通讯作者：
C. Paterson
通过红外和近红外拉曼光谱对人体晶状体脂质烃链的年龄和区域结构进行表征
DOI：
--
发表时间：
1998
期刊：
影响因子：
--
作者：
D. Borchman;Y. Ozaki;O. P. Lamba;W. Byrdwell;M. Yappert
通讯作者：
M. Yappert
内部钙对晶状体生理学的细胞毒性作用：综述。
影响因子：
2
作者：
Hightower,KR
通讯作者：
Hightower,KR
晶状体中的钙传输。
影响因子：
4.4
作者：
Hightower,KR;Leverenz,V;Reddy,VN
通讯作者：
Reddy,VN