High-speed particle tracking in microscopy using SPAD image sensors
High-speed particle tracking in microscopy using SPAD image sensors
复制标题
使用 SPAD 图像传感器在显微镜中进行高速粒子跟踪
DOI：
10.1117/12.2290199
复制
发表时间：
2018
期刊：
影响因子：
--
通讯作者：
Green A
中科院分区：
文献类型：
--
作者：
Green A
单光子雪崩二极管（spad）应用广泛，从荧光寿命成像显微镜（FLIM）到飞行时间（ToF） 3D成像。SPAD阵列正变得越来越成熟，它将SPAD的独特特性与宽视场相机配置相结合。传统上，SPAD阵列的光敏面积（填充因子）受到像素内数字电子器件的限制。然而，最近的设计表明，通过用简单的二进制像素和外部帧求和取代复杂的数字像素逻辑，填充因子可以大大增加。这种二进制SPAD阵列的一个显著优势是传感器提供的高帧率（bbb100 kfps），这为捕获超快速时间动态开辟了新的可能性，例如生命科学细胞成像。在这项工作中，我们考虑使用新的二进制SPAD阵列在高速粒子跟踪显微镜。我们展示了荧光微球在高帧率下进行布朗运动和细胞内囊泡动力学的跟踪。因此，我们展示了二进制SPAD阵列如何在细胞间通信、细胞运输和细胞信号传导等领域的活细胞成像中提供重要的进展。
Single photon avalanche diodes (SPADs) are used in a wide range of applications, from fluorescence lifetime imaging microscopy (FLIM) to time-of-flight (ToF) 3D imaging. SPAD arrays are becoming increasingly established, combining the unique properties of SPADs with widefield camera configurations. Traditionally, the photosensitive area (fill factor) of SPAD arrays has been limited by the in-pixel digital electronics. However, recent designs have demonstrated that by replacing the complex digital pixel logic with simple binary pixels and external frame summation, the fill factor can be increased considerably. A significant advantage of such binary SPAD arrays is the high frame rates offered by the sensors (>100kFPS), which opens up new possibilities for capturing ultra-fast temporal dynamics in, for example, life science cellular imaging. In this work we consider the use of novel binary SPAD arrays in high-speed particle tracking in microscopy. We demonstrate the tracking of fluorescent microspheres undergoing Brownian motion, and in intra-cellular vesicle dynamics, at high frame rates. We thereby show how binary SPAD arrays can offer an important advance in live cell imaging in such fields as intercellular communication, cell trafficking and cell signaling.
影响因子：
3.1
作者：
Antolovic, Ivan Michel;Burri, Samuel;Charbon, Edoardo
通讯作者：
Charbon, Edoardo