Longitudinal morphological and functional characterization of human heart organoids using optical coherence tomography.
Longitudinal morphological and functional characterization of human heart organoids using optical coherence tomography.
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DOI：
10.1016/j.bios.2022.114136
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发表时间：
2022-07-01
影响因子：
12.6
通讯作者：
中科院分区：
文献类型：
--
作者：
类器官作为研究器官发育、疾病机制和药物发现的体外模型发挥着越来越重要的作用。类器官是自组织的类器官三维 (3D) 细胞培养物，可开发器官特异性细胞类型和功能。最近，三个小组独立开发了由人类多能干细胞（hPSC）自组装的人类心脏类器官（hHO）。在这项研究中，我们利用定制的谱域光学相干断层扫描 (SD-OCT) 系统来表征 hHO 的生长。通过 OCT 和钙成像观察 hHO 的腔室结构和搏动模式的发育。我们展示了 OCT 以快速、无标签和非破坏性方式生成 3D 图像的能力。 hHO 形成不同大小的空腔，早在分化的第 4 天就观察到复杂的互连。 hHOs 模型和 OCT 成像系统作为研究心脏发育和疾病机制的体外平台显示出有前景的见解。
Organoids play an increasingly important role as in vitro models for studying organ development, disease mechanisms, and drug discovery. Organoids are self-organizing, organ-like three-dimensional (3D) cell cultures developing organ-specific cell types and functions. Recently, three groups independently developed self-assembling human heart organoids (hHOs) from human pluripotent stem cells (hPSCs). In this study, we utilized a customized spectral-domain optical coherence tomography (SD-OCT) system to characterize the growth of hHOs. Development of chamber structures and beating patterns of the hHOs were observed via OCT and calcium imaging. We demonstrated the capability of OCT to produce 3D images in a fast, label-free, and non-destructive manner. The hHOs formed cavities of various sizes, and complex interconnections were observed as early as on day 4 of differentiation. The hHOs models and the OCT imaging system showed promising insights as an in vitro platform for investigating heart development and disease mechanisms.
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影响因子：
4.6
作者：
Deloria, Abigail J.;Haider, Sandra;Haindl, Richard
通讯作者：
Haindl, Richard
影响因子：
14
作者：
Li RA;Keung W;Cashman TJ;Backeris PC;Johnson BV;Bardot ES;Wong AOT;Chan PKW;Chan CWY;Costa KD
通讯作者：
Costa KD
影响因子：
11.2
作者：
Huang Y;Wang S;Guo Q;Kessel S;Rubinoff I;Chan LL;Li P;Liu Y;Qiu J;Zhou C
通讯作者：
Zhou C
影响因子：
7.7
作者：
Dye BR;Hill DR;Ferguson MA;Tsai YH;Nagy MS;Dyal R;Wells JM;Mayhew CN;Nattiv R;Klein OD;White ES;Deutsch GH;Spence JR
通讯作者：
Spence JR
影响因子：
5.5
作者：
Lewis-Israeli YR;Wasserman AH;Aguirre A
通讯作者：
Aguirre A