Purity and yield of collagen extracted from southern catfish (Silurus meridionalis Chen) skin through improved pretreatment methods
Purity and yield of collagen extracted from southern catfish (Silurus meridionalis Chen) skin through improved pretreatment methods
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通过改进的预处理方法从南方鲶鱼（Silurus meridionalis Chen）皮肤中提取胶原蛋白的纯度和产量
DOI：
10.1080/10942912.2017.1291677
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发表时间：
2017-04
影响因子：
2.9
通讯作者：
Guoying Li
中科院分区：
文献类型：
--
作者：
Songcheng Xu;Huan Yang;Lirui Shen;Guoying Li
水产品加工副产品的经济和可持续利用已受到相当大的关注。工业过程中丢弃的大量鱼皮可以作为胶原蛋白提取的来源。本研究通过改进前处理方法，从南方鲇鱼皮中提取高纯度、高收率的胶原蛋白。多种脱脂方法均能有效地去除鱼皮中的脂肪，脱脂率最高可达90.24%。电泳、羟脯氨酸含量和提取率分析结果表明，在0.5%过氧化氢溶液（pH 10）中脱色的脱脂皮胶原蛋白纯度高，得率最高（湿重23.14%，干重78.57%）。组织学观察表明，预处理后鱼皮的非胶原性物质被有效去除，纤维疏松。氨基酸分析、紫外光谱、傅立叶变换红外光谱和热稳定性分析表明，在最佳预处理条件下从鱼皮中提取的胶原蛋白为I型胶原蛋白。我们的方法为最佳的预处理从鱼皮中提取的胶原蛋白后，脱色和脱色程序导致提高胶原蛋白的纯度和产量。
ABSTRACT The economic and sustainable use of fish-processing by-products has received considerable attention. Large amounts of fish skin discarded by industry processes can be a source of collagen extraction. This study developed a method to extract collagen with high purity and yield from Southern catfish skins through improved pretreatment methods. Multiple degreasing methods effectively removed fat from fish skins and obtained a maximum degreasing rate (90.24%). The results of electrophoretic, hydroxyproline-content, and extraction-rate analyses revealed that the collagen extracted from defatted skin, which decoloured in 0.5% hydrogen peroxide solution (pH 10), exhibited higher purity and the highest yield (23.14% wet weight and 78.57% dry weight, respectively) as compared with other decolouration conditions. The histological appearance of pretreated fish skin indicated that the non-collagenous substances were removed effectively and the fibres loosened. Amino acid analysis, ultraviolet spectra, Fourier transform infrared (FTIR) spectroscopy, and thermal-stability analysis indicated that the collagen isolated from fish skin under optimal pretreated conditions was classified as type I. Our method for the optimal pretreatment of collagen extracted from fish skin following degreasing and decolouring procedures resulted in improved collagen purity and yield.
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