Biological Evaluation of Acellular Cartilaginous and Dermal Matrixes as Tissue Engineering Scaffolds for Cartilage Regeneration.
Biological Evaluation of Acellular Cartilaginous and Dermal Matrixes as Tissue Engineering Scaffolds for Cartilage Regeneration.
复制标题
脱细胞软骨和真皮基质作为软骨再生组织工程支架的生物学评价
DOI：
10.3389/fcell.2020.624337
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发表时间：
2020
影响因子：
5.5
通讯作者：
Cao Y
中科院分区：
文献类型：
--
作者：
Wang Y;Xu Y;Zhou G;Liu Y;Cao Y
脱细胞基质（AM）作为一种天然生物材料在组织工程中的应用越来越受到重视。脱细胞软骨基质（ACM）和脱细胞真皮基质（ADM）是软骨组织工程中应用最广泛的两种基质。然而，对于这些AMs中哪一种实现了最佳的软骨再生，特别是在免疫能力强的大型动物中，仍然存在争议。在本研究中，我们采用冷冻干燥的方法制备了多孔的ADM和ACM支架，并证实ADM比ACM具有更大的孔径。通过与山羊耳软骨细胞的再定殖和体外培养，与ACM相比，ADM支架具有更高的细胞粘附率、更均匀的软骨分布和新软骨形成。此外，定量聚合酶链反应（qPCR）显示，ADM组软骨相关基因ACAN、COLIIA1、SOX9的表达明显高于ACM组。此外，在山羊皮下植入后，组织学评价表明，与ACM相比，ADM获得了更稳定和成熟的软骨，这一点通过湿重、体积、DNA、GAG、总胶原和胶原II含量等定量数据得到证实。此外，免疫评估表明，与ACM相比，ADM引起的免疫应答较低，这一点通过qPCR和CD3、CD68和TUNEL的免疫组织化学分析得到了证实。综上所述，我们的结果表明ADM是一种更适合软骨再生的AM，可以用于免疫功能良好的大型动物的软骨再生。
An acellular matrix (AM) as a kind of natural biomaterial is gaining increasing attention in tissue engineering applications. An acellular cartilaginous matrix (ACM) and acellular dermal matrix (ADM) are two kinds of the most widely used AMs in cartilage tissue engineering. However, there is still debate over which of these AMs achieves optimal cartilage regeneration, especially in immunocompetent large animals. In the current study, we fabricated porous ADM and ACM scaffolds by a freeze-drying method and confirmed that ADM had a larger pore size than ACM. By recolonization with goat auricular chondrocytes and in vitro culture, ADM scaffolds exhibited a higher cell adhesion rate, more homogeneous chondrocyte distribution, and neocartilage formation compared with ACM. Additionally, quantitative polymerase chain reaction (qPCR) indicated that expression of cartilage-related genes, including ACAN, COLIIA1, and SOX9, was significantly higher in the ADM group than the ACM group. Furthermore, after subcutaneous implantation in a goat, histological evaluation showed that ADM achieved more stable and matured cartilage compared with ACM, which was confirmed by quantitative data including the wet weight, volume, and contents of DNA, GAG, total collagen, and collagen II. Additionally, immunological assessment suggested that ADM evoked a low immune response compared with ACM as evidenced by qPCR and immunohistochemical analyses of CD3 and CD68, and TUNEL. Collectively, our results indicate that ADM is a more suitable AM for cartilage regeneration, which can be used for cartilage regeneration in immunocompetent large animals.
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影响因子：
3.3
作者：
Schwarz, Silke;Elsaesser, Alexander F.;Rotter, Nicole
通讯作者：
Rotter, Nicole
影响因子：
4.1
作者：
Schwarz, Silke;Koerber, Ludwig;Rotter, Nicole
通讯作者：
Rotter, Nicole
影响因子：
--
作者：
Rotter, N;Ung, F;Bonassar, LJ
通讯作者：
Bonassar, LJ
影响因子：
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作者：
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通讯作者：
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影响因子：
5.6
作者：
Ma, Anlun;Jiang, Li;Chen, Huifang
通讯作者：
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