Interaction of caveolin-1 with Ku70 inhibits Bax-mediated apoptosis.
Interaction of caveolin-1 with Ku70 inhibits Bax-mediated apoptosis.
复制标题
DOI：
10.1371/journal.pone.0039379
复制
发表时间：
2012
期刊：
影响因子：
3.7
通讯作者：
Galbiati F
中科院分区：
文献类型：
--
作者：
Zou H;Volonte D;Galbiati F
Caveolin-1, the structural protein component of caveolae, acts as a scaffolding protein that functionally regulates signaling molecules. We show that knockdown of caveolin-1 protein expression enhances chemotherapeutic drug-induced apoptosis and inhibits long-term survival of colon cancer cells. In vitro studies demonstrate that caveolin-1 is a novel Ku70-binding protein, as shown by the binding of the scaffolding domain of caveolin-1 (amino acids 82–101) to the caveolin-binding domain (CBD) of Ku70 (amino acids 471–478). Cell culture data show that caveolin-1 binds Ku70 after treatment with chemotherapeutic drugs. Mechanistically, we found that binding of caveolin-1 to Ku70 inhibits the chemotherapeutic drug-induced release of Bax from Ku70, activation of Bax, translocation of Bax to mitochondria and apoptosis. Potentiation of apoptosis by knockdown of caveolin-1 protein expression is greatly reduced in the absence of Bax expression. Finally, we found that overexpression of wild type Ku70, but not a mutant form of Ku70 that cannot bind to caveolin-1 (Ku70 Φ→A), limits the chemotherapeutic drug-induced Ku70/Bax dissociation and apoptosis. Thus, caveolin-1 acts as an anti-apoptotic protein in colon cancer cells by binding to Ku70 and inhibiting Bax-dependent cell death.
登录
查看更多内容
影响因子：
8
作者：
Hasenjäger, A;Gillissen, B;Daniel, PT
通讯作者：
Daniel, PT
DOI：
10.1084/jem.186.6.921
发表时间：
1997-09-15
期刊：
The Journal of experimental medicine
影响因子：
--
作者：
Ouyang H;Nussenzweig A;Kurimasa A;Soares VC;Li X;Cordon-Cardo C;Li Wh;Cheong N;Nussenzweig M;Iliakis G;Chen DJ;Li GC
通讯作者：
Li GC
影响因子：
16
作者：
Cohen, HY;Lavu, S;Sinclair, DA
通讯作者：
Sinclair, DA
影响因子：
4.8
作者：
Couet, J;Li, SW;Lisanti, MP
通讯作者：
Lisanti, MP
影响因子：
3.6
作者：
Kim, HA;Kim, KH;Lee, RA
通讯作者：
Lee, RA