Does egg deposition by herbivorous pine sawflies affect transcription of sesquiterpene synthases in pine?
Does egg deposition by herbivorous pine sawflies affect transcription of sesquiterpene synthases in pine?
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草食松树锯片的卵沉积会影响松树中倍半萜合酶的转录吗？
DOI：
10.1007/s00425-008-0747-8
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发表时间：
2008-08
期刊：
影响因子：
4.3
通讯作者：
Schmidt, Axel
中科院分区：
文献类型：
--
作者：
Koepke, Diana;Schroeder, Roland;Fischer, Hanna M.;Gershenzon, Jonathan;Hilker, Monika;Schmidt, Axel
众所周知，苏格兰松（Pinus sylvestris；松科，松属）通过改变其萜类挥发性混合物来防御草食性锯蝇的产卵。产卵诱导的松树气味吸引了杀死锯蝇卵的卵寄生物。在这里，我们通过提取产卵诱导的P. sylvestris的mRNA来研究锯蝇卵沉积是否激活了编码松萜类合成酶的基因。分离到三种新的倍半萜合成酶，分别为PsTPS 1、PsTPS 2和PsTPS 3，在大肠杆菌中进行了异源表达，主要产物为(E)-β-石竹烯和α-葎草烯（PsTPS 1）、1（10）、5-germacradiene-4-ol （PsTPS 2）和长叶烯和α-长叶蒎烯（PsTPS 3）。定量RT-PCR分析显示，在诱导产卵的、对寄生蜂有吸引力的枝条中，pps 1和pps 2的转录水平显著高于非吸引的、人为破坏的枝条。因此，我们的研究结果证明了对卵子沉积的特定转录反应，不同于人工损伤引起的转录反应。pps 3的转录本不随卵沉积而改变。pps 1、pps 2和pps 3的转录水平也与卵沉积后的时间有关，因为松树气味在卵沉积后72小时才对寄生蜂产生吸引力。pps 1和pps 2的转录率仅在卵沉积72 h后显著增强，因此与气味吸引的时间相匹配，而pps 3的转录率在卵沉积后的任何时间内均未检测到增强。讨论了产卵诱导倍半萜合成酶转录物增加的生态学意义。本文的在线版本（doi:10.1007/s00425-008-0747-8）包含补充材料，仅供授权用户使用。
Scots pine (Pinus sylvestris; Pinaceae, Pinales) is known to defend against egg deposition by herbivorous sawflies by changing its terpenoid volatile blend. The oviposition-induced pine odor attracts egg parasitoids that kill the sawfly eggs. Here, we investigated whether sawfly egg deposition activates genes encoding pine terpene synthases by extracting mRNA from oviposition-induced P. sylvestris. Three new sesquiterpene synthases, PsTPS 1, PsTPS 2, and PsTPS 3, were isolated that were shown on heterologous expression in Escherichia coli to produce (E)-β-caryophyllene and α-humulene (PsTPS 1), 1(10),5-germacradiene-4-ol (PsTPS 2), and longifolene and α-longipinene (PsTPS 3) as their principal products. Quantitative RT-PCR analyses revealed that transcript levels of PsTPS 1 and PsTPS 2 were significantly higher in oviposition-induced twigs that were attractive to the parasitoids than in non-attractive, artificially damaged twigs. Thus, our results demonstrate a specific transcription response to egg deposition, distinct from that caused by artificial wounding. Transcripts of PsTPS 3 did not change in response to egg deposition. The transcript levels of PsTPS 1, PsTPS 2, and PsTPS 3 were also determined in relation to time after egg deposition, since pine odor is attractive to the parasitoid only 72 h after egg deposition. Transcription rates of PsTPS 1 and PsTPS 2 were significantly enhanced only 72 h after egg deposition, thus matching the timing of odor attractiveness, while for PsTPS 3, enhanced transcription was not detected at any time period studied after egg deposition. The ecological significance of the oviposition-induced increase of sesquiterpene synthase transcripts is discussed. The online version of this article (doi:10.1007/s00425-008-0747-8) contains supplementary material, which is available to authorized users.
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