Identification of Natural CRISPR Systems and Targets in the Human Microbiome.
Identification of Natural CRISPR Systems and Targets in the Human Microbiome.
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人类微生物组中天然CRISPR系统和靶标的鉴定。
DOI：
10.1016/j.chom.2020.10.010
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发表时间：
2021-01-13
影响因子：
30.3
通讯作者：
Huttenhower C
中科院分区：
文献类型：
--
作者：
Münch PC;Franzosa EA;Stecher B;McHardy AC;Huttenhower C
Many bacteria resist invasive DNA by incorporating sequences into CRISPR loci, which enable sequence-specific degradation. CRISPR systems have been well-studied from isolate genomes, but culture-independent metagenomics provides a new window into their diversity. We profiled CRISPR loci and cas genes in the body-wide human microbiome using 2,355 metagenomes, yielding functional and taxonomic profiles for 2.9 million spacers by aligning the spacer content to each sample’s metagenome and corresponding gene families. Spacer and repeat profiles agree qualitatively with those from isolate genomes but expand their diversity by approximately 13-fold, with the highest spacer load present in the oral microbiome. The taxonomy of spacer sequences parallels that of their source community, with functional targets enriched for viral elements. When coupled with cas gene systems, CRISPR–Cas subtypes are highly site- and taxon-specific. Our analysis provides a comprehensive collection of natural CRISPR–cas loci and targets in the human microbiome. In this study, Münch et al. carried out a taxonomic and functional characterisation of CRISPR systems in 2,355 human microbiomes. Together with quantification of cas gene abundance, this informs the potential roles of CRISPR/Cas systems and their targets, as well as evolutionary properties and principles of bacteria-virus relationships.
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影响因子：
5.8
作者：
Gu, Zuguang;Eils, Roland;Schlesner, Matthias
通讯作者：
Schlesner, Matthias
影响因子：
4.4
作者：
Gogleva AA;Gelfand MS;Artamonova II
通讯作者：
Artamonova II
DOI：
10.3390/life5021333
发表时间：
2015-05-05
期刊：
Life (Basel, Switzerland)
影响因子：
--
作者：
Horz HP
通讯作者：
Horz HP
影响因子：
11
作者：
Abeles, Shira R.;Robles-Sikisaka, Refugio;Pride, David T.
通讯作者：
Pride, David T.
影响因子：
56.9
作者：
Heithoff, DM;Sinsheimer, RL;Mahan, MJ
通讯作者：
Mahan, MJ