Characterization of monoclonal antibodies recognizing different fragments of cartilage oligomeric matrix protein in human body fluids.
Characterization of monoclonal antibodies recognizing different fragments of cartilage oligomeric matrix protein in human body fluids.
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识别人体液中软骨寡聚基质蛋白不同片段的单克隆抗体的表征。
DOI：
10.1006/abbi.1997.9941
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发表时间：
1997
期刊：
影响因子：
--
通讯作者：
Thonar,EJ
中科院分区：
文献类型：
--
作者：
Vilim,V;Lenz,ME;Vytasek,R;Masuda,K;Pavelka,K;Kuettner,KE;Thonar,EJ
软骨寡聚基质蛋白（COMP）是一种高分子量糖蛋白，在关节软骨中以高浓度存在。最近的研究表明，通过酶联免疫吸附测定（ELISA）测量的抗原性COMP的关节液和血清水平提供了关于关节疾病中软骨基质中发生的代谢变化的重要信息，所述酶联免疫吸附测定（ELISA）使用抗牛COMP的多克隆抗血清。在这份报告中，我们描述了三种单克隆抗体（mAb）对人COMP的特异性及其在定量体液中抗原COMP片段中的有用性。其中两种mAb（16-F12和18-G3）识别COMP的寡聚体和单体形式，但第三种mAb（17-C10）仅与前者呈阳性反应。用胰蛋白酶预消化长达6 h的人COMP的免疫印迹显示，三种mAb分别针对30 kDa（16-F12）、25 kDa（17-C10）和40 kDa以及30 kDa（18-G3）的小胰蛋白酶片段上鉴定的不同表位。这些抗体还识别人类病理性滑液中不同的片段模式。这在中等大小的片段（16-F12：90和110 kDa; 17-C10：70和90 kDa; 18-G3：从70至130 kDa的多达5个条带）的情况下尤其引人注目。用mAb 16-F12和17-C10开发的竞争性间接抑制ELISA揭示了这些抗体的特异性的进一步差异。因此，虽然mAb 16-F12仅可用于定量人滑液和血清中的抗原性COMP，但mAb 17-C10在分析犬和马滑液以及犬血清时也是有用的。对骨关节炎和类风湿性关节炎患者滑液样本的分析结果提供了初步证据，支持以下论点：测量体液中这些mAb识别的不同COMP表位可用于关节疾病患者的临床评估。
Cartilage oligomeric matrix protein (COMP) is a high-molecular-weight glycoprotein found at a high concentration in articular cartilage. Recent studies have shown that the joint fluid and serum levels of antigenic COMP, measured by an enzyme-linked immunosorbent assay (ELISA) which uses a polyclonal antiserum raised against bovine COMP, provide important information about metabolic changes occurring in the cartilage matrix in joint disease. In this report, we describe the specificity of three monoclonal antibodies (mAbs) to human COMP and their usefulness in quantifying antigenic COMP fragments in body fluids. Two of the mAbs (16-F12 and 18-G3) recognized both oligomeric and monomeric forms of COMP, but the third (17-C10) reacted positively only with the former. Immunoblots of human COMP, predigested with trypsin for up to 6 h, showed that the three mAbs are directed against different epitopes identified on small tryptic fragments of 30 kDa (16-F12), 25 kDa (17-C10), and 40 kDa as well as 30 kDa (18-G3), respectively. The antibodies also recognized a different pattern of fragments in human pathological synovial fluids. This was particularly striking in the case of the medium size fragments (16-F12: 90 and 110 kDa; 17-C10: 70 and 90 kDa; 18-G3: up to five bands from 70 to 130 kDa). Competitive indirect inhibition ELISAs developed with mAbs 16-F12 and 17-C10 revealed further differences in the specificities of these antibodies. Thus, while mAb 16-F12 can be used only to quantify antigenic COMP in human synovial fluid and serum, mAb 17-C10 is useful in addition when analyzing canine and horse synovial fluid as well as canine serum. The results of analyses of synovial fluid samples from patients with osteoarthritis and rheumatoid arthritis provided preliminary evidence in support of the contention that measurement of the different COMP epitopes recognized by these mAbs in body fluids could prove useful in the clinical assessment of patients with joint disease.
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从人关节软骨联合提取物中分离出的蛋白多糖的表征。
影响因子：
4.1
作者：
V. Vilím;A. Fosang
通讯作者：
A. Fosang
DOI：
10.1111/j.1432-1033.1994.tb19070.x
发表时间：
1994-08-01
期刊：
EUROPEAN JOURNAL OF BIOCHEMISTRY
影响因子：
--
作者：
DICESARE, PE;MORGELIN, M;PAULSSON, M
通讯作者：
PAULSSON, M
影响因子：
4.4
作者：
NEWTON, G;WEREMOWICZ, S;LAWLER, J
通讯作者：
LAWLER, J
狗膝关节前交叉韧带横断后，血清透明质酸水平快速持续升高。
DOI：
--
发表时间：
1995
期刊：
The Journal of rheumatology
影响因子：
--
作者：
Manicourt,DH;Cornu,O;Lenz,ME;Druetz-vanEgeren,A;Thonar,EJ
通讯作者：
Thonar,EJ
两种软骨基质蛋白的血清浓度反映了复发性多软骨炎中软骨更新的不同方面。
影响因子：
--
作者：
T. Saxne;D. Heinegård
通讯作者：
D. Heinegård