Electrospun Fibrinogen-Polydioxanone Composite Matrix: Potential for in Situ Urologic Tissue Engineering
Electrospun Fibrinogen-Polydioxanone Composite Matrix: Potential for in Situ Urologic Tissue Engineering
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电纺纤维蛋白原-聚二氧环己酮复合基质：原位泌尿组织工程的潜力
DOI：
10.1177/155892500800300204
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发表时间：
2008
影响因子：
2.9
通讯作者：
G. Bowlin
中科院分区：
文献类型：
--
作者：
Michael C. McManus;S. Sell;W. Bowen;H. Koo;D. Simpson;G. Bowlin
我们的目的是证明电纺纤维蛋白原-PDO（聚对二氧环己酮）复合支架将保留纤维蛋白原的上级细胞相互作用，同时产生具有直接植入所需的功能强度的产品。纤维蛋白原-PDO复合支架以PDO比率为0%（纯纤维蛋白原）、10%、20%、30%、40%、50%和100%（纯PDO）进行静电纺丝，并使用标准方法进行消毒。将支架用人BSM（膀胱平滑肌细胞）接种，并与每周两次的培养基更换一起孵育。在第7、14和21天取出样品，通过胶原测定、扫描电子显微镜和组织学进行评价。细胞接种和培养表明，人BSM容易迁移，并重塑电纺纤维蛋白原-PDO复合支架与天然胶原蛋白的沉积。随着纤维蛋白原浓度的增加，细胞迁移和胶原沉积增加，而支架完整性随着PDO浓度的增加而增加。电纺纤维蛋白原-PDO复合结构促进人BSM的快速细胞向内生长，同时保持结构完整性。可以调节纤维蛋白原与PDO的比率以实现特定组织工程应用所需的期望性质。我们的最终目标是利用这一创新的生物材料技术，生产一种无细胞的，生物可吸收的产品，使原位组织再生。虽然还有很多工作要做，这些初步的研究结果表明，纤维蛋白原-PDO复合支架值得进一步研究。
Our objective is to demonstrate an electrospun fibrinogen-PDO (polydioxanone) composite scaffold will retain the superior cellular interaction of fibrinogen while producing a product with the functional strength needed for direct implantation. Fibrinogen-PDO composite scaffolds were electrospun with PDO ratios of 0% (pure fibrinogen), 10%, 20%, 30%, 40%, 50% and 100% (pure PDO) and disinfected using standard methods. Scaffolds were seeded with human BSM (bladder smooth muscle cells) and incubated with twice weekly media changes. Samples were removed at 7, 14 and 21 days for evaluation by collagen assay, scanning electron microscopy and histology. Cell seeding and culture demonstrated human BSM readily migrate throughout and remodel electrospun fibrinogen-PDO composite scaffolds with deposition of native collagen. Cell migration and collagen deposition increased with increasing fibrinogen concentration while scaffold integrity increased with increasing PDO concentration. Electrospun fibrinogen-PDO composite structures promote rapid cellular in-growth by human BSM while maintaining structural integrity. The fibrinogen to PDO ratio can be adjusted to achieve the desired properties required for a specific tissue engineering application. Our ultimate objective is to utilize this innovative biomaterial technology to produce an acellular, bioresorbable product that enables in situ tissue regeneration. While there is still much work to be done, these initial findings indicate fibrinogen-PDO composite scaffolds deserve further investigation.
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影响因子：
14
作者：
Greisler, HP;Gosselin, C;Kim, DU
通讯作者：
Kim, DU
影响因子：
6.5
作者：
CLARK, RAF;LANIGAN, JM;COLVIN, RB
通讯作者：
COLVIN, RB
P-选择素和伤口愈合。
DOI：
--
发表时间：
1993
期刊：
Behring Institute Mitteilungen
影响因子：
--
作者：
Erban,JK
通讯作者：
Erban,JK
影响因子：
2
作者：
WEIGEL, PH;FULLER, GM;LEBOEUF, RD
通讯作者：
LEBOEUF, RD