Probing the interaction of Arg9Cys mutated phospholamban with phospholipid bilayers by solid-state NMR spectroscopy.
Probing the interaction of Arg9Cys mutated phospholamban with phospholipid bilayers by solid-state NMR spectroscopy.
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DOI：
10.1016/j.bbamem.2013.07.003
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发表时间：
2013-11
期刊：
影响因子：
--
通讯作者：
Lorigan GA
中科院分区：
文献类型：
--
作者：
Yu X;Lorigan GA
受磷蛋白（PLB）是一种由52个氨基酸组成的膜蛋白，它与肌浆网Ca 2 + ATP酶（SERCA）相互作用，并有助于调节Ca 2+流动。PLB抑制SERCA损伤Ca ~（2+）转运。当PLB磷酸化时，抑制作用可被解除。Arg 9至Cys（R9 C）突变是具有降低的抑制效力的功能缺失突变。利用31 P和2 H固体核磁共振技术研究了R9 C PLB对多层囊泡（MLV）膜表面和疏水区动力学的影响。31 P NMR光谱表明，与磷酸化PLB（P-PLB）一样，突变的R9 C-PLB蛋白与野生型PLB（WT-PLB）相比，与脂质双层头基的相互作用显著减少。与P-PLB类似，R9 C-PLB略微降低脂质头基区域中的31 P T1值。当与WT-PLB相比时，R9 C-PLB和P-PLB的2 H核沿着脂质酰基链的2 H SCD顺序参数降低不太显著。结果表明，R9 C-PLB与膜表面和疏水区的相互作用比WT-PLB少。R9 C-PLB的胞质结构域从膜表面的分离可能与其功能丧失有关。
Phospholamban (PLB) is a 52 amino acid integral membrane protein that interacts with the sarcoplasmic reticulum Ca2+ ATPase (SERCA) and helps to regulate Ca2+ flow. PLB inhibits SERCA impairing Ca2+ translocation. The inhibition can be relieved upon phosphorylation of PLB. The Arg9 to Cys (R9C) mutation is a loss of function mutation with reduced inhibitory potency. The effect R9C PLB has on the membrane surface and the hydrophobic region dynamics was investigated by 31P and 2H solid-state NMR spectroscopy in multilamellar vesicles (MLVs). The 31P NMR spectra indicate that, like the phosphorylated PLB (P-PLB), the mutated R9C-PLB protein has significantly less interaction with the lipid bilayer headgroup when compared to wild-type PLB (WT-PLB). Similar to P-PLB, R9C-PLB slightly decreases 31P T1 values in the lipid headgroup region. 2H SCD order parameters of 2H nuclei along the lipid acyl chain decrease less dramatically for R9C-PLB and P-PLB when compared to WT-PLB. The results suggest that R9C-PLB interacts less with the membrane surface and hydrophobic region than WT-PLB. Detachment of the cytoplasmic domain of R9C-PLB from the membrane surface could be related to its loss of function.
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影响因子：
3.4
作者：
Chu, Shidong;Coey, Aaron T.;Lorigan, Gary A.
通讯作者：
Lorigan, Gary A.
影响因子：
3.2
作者：
Dave, PC;Tiburu, EK;Lorigan, GA
通讯作者：
Lorigan, GA
影响因子：
3.4
作者：
Huster, D;Yao, YL;Hong, M
通讯作者：
Hong, M
影响因子：
5.6
作者：
Gustavsson, Martin;Traaseth, Nathaniel J.;Veglia, Gianluigi
通讯作者：
Veglia, Gianluigi
Phospholamban 突变体与野生型竞争活细胞中的 SERCA 结合。
DOI：
10.1016/j.bbrc.2012.02.125
发表时间：
2012-04-06
影响因子：
3.1
作者：
Gruber SJ;Haydon S;Thomas DD
通讯作者：
Thomas DD