Systematic prevention of bubble formation and accumulation for long-term culture of pancreatic islet cells in microfluidic device.
Systematic prevention of bubble formation and accumulation for long-term culture of pancreatic islet cells in microfluidic device.
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DOI：
10.1007/s10544-011-9618-3
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发表时间：
2012-04
影响因子：
2.8
通讯作者：
Oberholzer, Jose
中科院分区：
文献类型：
--
作者：
Wang, Yong;Lee, Dongyoung;Zhang, Lisa;Jeon, Hyojin;Mendoza-Elias, Joshua E.;Harvat, Tricia A.;Hassan, Sarah Z.;Zhou, Amanda;Eddington, David T.;Oberholzer, Jose
在微流控系统中可靠的长期细胞培养受到气泡形成和积累的限制。在这项研究中，我们开发了一种气泡去除系统，能够以一致和可靠的方式捕获和排放气泡。结合PDMS（Polydimethylsiloxane）亲水表面处理和真空灌流技术，构建了一种配备气泡捕获器的微流控灌流系统，成功应用于小鼠胰岛的长期培养，无气泡形成和流动中断。除了显示正常的细胞活力和胰岛形态外，培养后的胰岛还表现出正常的胰岛素分泌动力学、细胞内钙信号传导以及响应葡萄糖挑战的线粒体电位变化。这种设计可以很容易地适应其他微流体系统，由于其简单的设计，易于制造，和便携性。
Reliable long-term cell culture in microfluidic system is limited by air bubble formation and accumulation. In this study, we developed a bubble removal system capable of both trapping and discharging air bubbles in a consistent and reliable manner. Combined with PDMS (Polydimethylsiloxane) hydrophilic surface treatment and vacuum filling, a microfluidic perifusion system equipped with the bubble trap was successfully applied for long-term culture of mouse pancreatic islets with no bubble formation and no flow interruption. In addition to demonstrating normal cell viability and islet morphology, post-cultured islets exhibited normal insulin secretion kinetics, intracellular calcium signaling, and changes in mitochondrial potentials in response to glucose challenge. This design could be easily adapted by other microfluidic systems due to its simple design, ease of fabrication, and portability.
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影响因子：
7.4
作者：
Monahan, J;Gewirth, AA;Nuzzo, RG
通讯作者：
Nuzzo, RG
影响因子：
3.9
作者：
Xu, J. H.;Li, S. W.;Luo, G. S.
通讯作者：
Luo, G. S.
影响因子：
7.4
作者：
Roper, MG;Shackman, JG;Kennedy, RT
通讯作者：
Kennedy, RT
影响因子：
6.1
作者：
Shackman, JG;Dahlgren, GM;Kennedy, RT
通讯作者：
Kennedy, RT
DOI：
10.1007/bf02581331
发表时间：
1990-07-01
期刊：
ACTA DIABETOLOGICA LATINA
影响因子：
--
作者：
RICORDI, C;GRAY, DWR;LACY, PE
通讯作者：
LACY, PE