A scalable phenotyping approach for female floral organ development and senescence in the absence of pollination in wheat
A scalable phenotyping approach for female floral organ development and senescence in the absence of pollination in wheat
复制标题
小麦无授粉情况下雌性花器官发育和衰老的可扩展表型分析方法
DOI:
10.1101/2022.04.01.486528
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发表时间:
2022
期刊:
影响因子:
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通讯作者:
Millan-Blanquez M
中科院分区:
文献类型:
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作者:
Millan-Blanquez M
In the absence of pollination, female reproductive organs senesce, leading to an irrevocable loss in the reproductive potential of the flower, which directly affects seed set. In self-pollinating crops like wheat (Triticum aestivum), the post-anthesis viability of unpollinated carpels has been overlooked, despite its importance for hybrid seed production systems. To advance our knowledge of carpel development in the absence of pollination, we created a high-throughput phenotyping approach to quantify stigma and ovary morphology. We demonstrate the suitability of the approach, which uses light-microscopy imaging and machine learning, for the analysis of floral organ traits in field-grown plants using fresh and fixed samples. We show that the unpollinated carpel undergoes a well-defined initial growth phase, followed by a peak phase in which stigma area reaches its maximum and the radial expansion of the ovary slows, and a final deterioration phase. These developmental dynamics were consistent across years and could be used to classify male-sterile cultivars. This phenotyping approach provides a new tool for examining carpel development, which we hope will advance research into female fertility of wheat.
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DOI:
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作者:
Elda R. Walker
通讯作者:
Elda R. Walker
DOI:
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发表时间:
1984
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影响因子:
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作者:
Y. Vercher;A. Molowny;C. López;J. García;J. Carbonell
通讯作者:
J. Carbonell
DOI:
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发表时间:
1980
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作者:
M. Molnár;B. Barnabás;E. Rajki
通讯作者:
E. Rajki
影响因子:
48
作者:
Falk, Thorsten;Mai, Dominic;Ronneberger, Olaf
通讯作者:
Ronneberger, Olaf
影响因子:
56.9
作者:
Appels, Rudi;Eversole, Kellye;Uauy, Cristobal
通讯作者:
Uauy, Cristobal