Diabetic Pathophysiology Enhances Inflammation during Extracorporeal Membrane Oxygenation in a Rat Model.
Diabetic Pathophysiology Enhances Inflammation during Extracorporeal Membrane Oxygenation in a Rat Model.
复制标题
糖尿病病理生理学在大鼠模型中增强体外膜氧合期间的炎症。
DOI：
10.3390/membranes11040283
复制
发表时间：
2021-04-11
期刊：
影响因子：
4.2
通讯作者：
Ikegami K
中科院分区：
文献类型：
--
作者：
Fujii Y;Abe T;Ikegami K
接受体外膜氧合（ECMO）的患者的全身炎症反应显著增加了ECMO相关的发病率和死亡率。近年来，2型糖尿病患者的数量有所增加，这类患者接受ECMO的数量也有所增加。2型糖尿病是ECMO并发症的高危因素。我们研究了ECMO对糖尿病大鼠ECMO模型炎症反应的影响。将28只大鼠分为4组：正常SHAM组（n = 7）、糖尿病SHAM组（n = 7）、正常ECMO组（n = 7）、糖尿病ECMO组（n = 7）。我们测量了血浆细胞因子、肿瘤坏死因子-α和白细胞介素-6的水平。采用体外循环大鼠模型检测天冬氨酸转氨酶（AST）、丙氨酸转氨酶（ALT）、乳酸脱氢酶（LDH）、血尿素氮（BUN）、肌酐（Cr）、肝型脂肪酸结合蛋白（L-FABP），以确定脏器损伤。此外，测量肺干湿重（W/D）比作为肺组织水肿的指标。采用苏木精-伊红（HE）和周期酸-甲基胺-银（PAM）染色对肾脏进行病理评价。糖尿病ECMO组细胞因子、AST、ALT、LDH、L-FABP水平显著升高，与其他组比较，在ECMO结束时达到最大值（p < 0.05）。此外，ECMO组（正常ECMO组和糖尿病ECMO组）肾组织苏木精-伊红染色和周期酸-甲基胺-银染色显示明显的损伤。此外，当比较正常ECMO组和糖尿病ECMO组时，糖尿病ECMO组出现严重的器官损伤。糖尿病ECMO组有明显的脏器损伤。这些数据表明，在动物模型中，糖尿病增加了ECMO期间促炎细胞因子的释放、肾脏损伤和肺水肿。
Systemic inflammatory responses in patients undergoing extracorporeal membrane oxygenation (ECMO) contribute significantly to ECMO-associated morbidity and mortality. In recent years, the number of type 2 diabetes mellitus patients has increased, and the number of these patients undergoing ECMO has also increased. Type 2 diabetes mellitus is a high-risk factor for complications during ECMO. We studied the effects of ECMO on inflammatory response in a diabetic rat ECMO model. Twenty-eight rats were divided into 4 groups: normal SHAM group (normal rats: n = 7), diabetic SHAM group (diabetic rats: n = 7), normal ECMO group (normal rats: n = 7), and diabetic ECMO group (diabetic rats: n = 7). We measured the plasma levels of cytokines, tumor necrosis factor-α, and interleukin-6. Aspartate aminotransferase (AST), alanine aminotransferase (ALT), lactate dehydrogenase (LDH), blood urea nitrogen (BUN), creatinine (Cr), and liver-type fatty acid binding protein (L-FABP) were examined in the rat cardiopulmonary bypass model to ascertain organ damage. In addition, the lung wet-to-dry weight (W/D) ratio was measured as an index of pulmonary tissue edema. A pathologic evaluation of kidneys was conducted by hematoxylin-eosin (HE) and periodic-acid-methenamine-silver (PAM) staining. In the diabetic ECMO group, levels of cytokines, AST, ALT, LDH, and L-FABP increased significantly, reaching a maximum at the end of ECMO in comparison with other groups (p < 0.05). In addition, hematoxylin-eosin and periodic acid-methenamine-silver staining of renal tissues showed marked injury in the ECMO group (normal ECMO and diabetic ECMO groups). Furthermore, when the normal ECMO and diabetic ECMO groups were compared, severe organ injury was seen in the diabetic ECMO group. There was remarkable organ injury in the diabetic ECMO group. These data demonstrate that diabetes enhances proinflammatory cytokine release, renal damage, and pulmonary edema during ECMO in an animal model.
登录
查看更多内容
影响因子：
3.3
作者：
Moledina DG;Parikh CR
通讯作者：
Parikh CR
DOI：
10.1016/j.jtcvs.2005.08.018
发表时间：
2005-12-01
影响因子：
6
作者：
Alex, J;Laden, G;Griffin, SC
通讯作者：
Griffin, SC
影响因子：
3
作者：
Goudeau, Jean-Jacques;Clermont, Gaelle;Girard, Claude
通讯作者：
Girard, Claude
影响因子：
4.6
作者：
Mahmood, Eitezaz;Jeganathan, Jelliffe;Matyal, Robina
通讯作者：
Matyal, Robina
影响因子：
2.4
作者：
Goncalves-Venade, Gabriela;Lacerda-Principe, Nuno;Paiva, Jose Artur
通讯作者：
Paiva, Jose Artur