Activation of orexin/hypocretin projections to basal forebrain and paraventricular thalamus by acute nicotine.
Activation of orexin/hypocretin projections to basal forebrain and paraventricular thalamus by acute nicotine.
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DOI：
10.1016/j.brainresbull.2008.09.014
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发表时间：
2008-12-16
影响因子：
3.8
通讯作者：
Fadel, Jim
中科院分区：
文献类型：
--
作者：
Pasumarthi, Ravi K.;Fadel, Jim
促食素/食欲素神经元外侧下丘脑/ perifornical区域项目的一系列不同的大脑区域,并对各种精神兴奋剂。有研究表明，全身给药尼古丁会激活食欲素神经元，这表明这种药物对觉醒和认知功能的影响可能与食欲素有关。基底前脑和丘脑背侧室旁核（PVT）都接受食欲素输入，并与觉醒、注意力和精神兴奋剂药物反应有关。然而，目前尚不清楚这些区域的食欲素输入是否被尼古丁等精神兴奋剂激活。本研究将逆行道示踪剂霍乱毒素B亚基（CTb）注入成年雄性大鼠基底前脑或PVT。7至10天后，动物接受急性全身给药（−）酒石酸氢尼古丁或对照物，2小时后安乐死。采用三标记免疫组织化学/免疫荧光法检测逆行标记的食欲素神经元中Fos的表达。尼古丁增加了投射到基底前脑和PVT的食欲素神经元中Fos的表达。在基底前脑CTb沉积后，逆行标记的食欲素神经元的外侧和内侧库的相对激活相似，但在CTb的PVT沉积后，内侧库的激活更为明显。我们的研究结果表明，食欲素输入基底前脑和PVT可能有助于尼古丁对觉醒和认知的影响，并进一步支持食欲素神经元中外侧分布的功能异质性的存在。
Orexin/hypocretin neurons of the lateral hypothalamus/perifornical area project to a diverse array of brain regions and are responsive to a variety of psychostimulant drugs. It has been shown that orexin neurons are activated by systemic nicotine adminstration suggesting a possible orexinergic contribution to the effects of this drug on arousal and cognitive function. The basal forebrain and paraventricular nucleus of the dorsal thalamus (PVT) both receive orexin inputs and have been implicated in arousal, attention and psychostimulant drug responses. However, it is unknown whether orexin inputs to these areas are activated by psychostimulant drugs such as nicotine. Here, we infused the retrograde tract tracer cholera toxin B subunit (CTb) into either the basal forebrain or PVT of adult male rats. Seven to 10 days later, animals received an acute systemic administration of (−) nicotine hydrogen tartrate or vehicle and were euthanized two hours later. Triple-label immunohistochemistry/immunofluorescence was used to detect Fos expression in retrogradely-labeled orexin neurons. Nicotine increased Fos expression in orexin neurons projecting to both basal forebrain and PVT. The relative activation in lateral and medial banks of retrogradely-labeled orexin neurons was similar following basal forebrain CTb deposits, but was more pronounced in the medial bank following PVT deposits of CTb. Our findings suggest that orexin inputs to the basal forebrain and PVT may contribute to nicotine effects on arousal and cognition and provide further support for the existence of functional heterogeneity across the medial-lateral distribution of orexin neurons.
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影响因子：
3.3
作者：
Fadel, J;Pasumarthi, R;Reznikov, LR
通讯作者：
Reznikov, LR
影响因子：
2.5
作者：
España, RA;Reis, KM;Berridge, CW
通讯作者：
Berridge, CW
影响因子：
16.2
作者：
Borgland, SL;Taha, SA;Bonci, A
通讯作者：
Bonci, A
影响因子：
3.3
作者：
España, RA;Baldo, BA;Berridge, CW
通讯作者：
Berridge, CW
影响因子：
2.3
作者：
Parsons, MP;Li, S;Kirouac, GJ
通讯作者：
Kirouac, GJ