An estradiol-inducible promoter enables fast, graduated control of gene expression in fission yeast.
An estradiol-inducible promoter enables fast, graduated control of gene expression in fission yeast.
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DOI：
10.1002/yea.3235
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发表时间：
2017-08
期刊：
影响因子：
--
通讯作者：
Rhind N
中科院分区：
文献类型：
--
作者：
Ohira MJ;Hendrickson DG;Scott McIsaac R;Rhind N
The fission yeast Schizosaccharomyces pombe lacks a diverse toolkit of inducible promoters for experimental manipulation. Available inducible promoters suffer from slow induction kinetics, limited control of expression levels and/or a requirement for defined growth medium. In particular, no S. pombe inducible promoter systems exhibit a linear dose response, which would allow expression to be tuned to specific levels. We have adapted a fast, orthogonal promoter system with a large dynamic range and a linear dose response, based on β-estradiol-regulated function of the human estrogen receptor, for use in S. pombe. We show that this promoter system, termed Z3EV, turns on quickly, can reach a maximal induction of 20 fold, and exhibits a linear dose response over its entire induction range, with few off target effects. We demonstrate the utility of this system by regulating the mitotic inhibitor Wee1 to create a strain in which cell size is regulated by β-estradiol concentration. This promoter system will be of great utility for experimentally regulating gene expression in fission yeast.
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