Specific production of eosinophil colony stimulating factor from sensitized T cells from a patient with allergic eosinophilia
Specific production of eosinophil colony stimulating factor from sensitized T cells from a patient with allergic eosinophilia
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过敏性嗜酸性粒细胞增多症患者的致敏 T 细胞特异性产生嗜酸性粒细胞集落刺激因子
DOI：
10.1111/j.1365-2141.1985.tb02967.x
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发表时间：
1985
影响因子：
6.5
通讯作者：
H. Shishido
中科院分区：
文献类型：
--
作者：
H. Enokihara;H. Hamaguchi;H. Sakamaki;Sakae Hazama;Kenji Saito;S. Furusawa;H. Shishido
总结。为了探讨嗜酸性粒细胞集落刺激因子(EO-CSF)独立于中性粒细胞集落刺激因子(N-CSF)产生的可能性，我们比较了在嗜酸性粒细胞增多症过敏性支气管肺曲霉菌病患者外周血白细胞中加入特定抗原曲霉菌提取物对EO-CSF和N-CSF产生的影响。以正常人非吞噬MN骨髓细胞为靶细胞，采用琼脂培养技术检测患者单个核细胞和T细胞条件培养液中EO-CSF和N-CSF活性。在患者的MN或T细胞培养中加入该特定抗原，可显著刺激EO-CSF的产生，但不能刺激N-CSF的产生，而患者的非T细胞和正常的MN或T细胞不受抗原刺激而产生两者。这些结果表明，EO-CSF是一个有别于N-CSF的因子，它的产生依赖于抗原特异性刺激的致敏T细胞的存在，这可能是该患者血中嗜酸性粒细胞增多的原因之一。
Summary. To explore the possibility that an eosinophil colony stimulating factor (EO‐CSF) is elaborated independently of neutrophil CSF (N‐CSF), we compared the effect on the production of EO‐CSF and N‐CSF of adding a specific antigen, an aspergillus extract, to peripheral blood leucocytes of an eosinophilic patient with allergic bronchopulmonary aspergillosis. Conditioned media prepared from the patient's mononuclear (MN) and T cells were assayed for EO‐CSF and N‐CSF activities by agar culture technique, using normal human nonphagocytic MN bone marrow cells as target cells. The addition of the specific antigen to the cultures of the patient's MN or T cells significantly stimulated the production of EO‐CSF, but not that of N‐CSF, while the patient's non‐T cells and normal MN or T cells were not stimulated by the antigen challenge to produce either CSF. These results suggest that EO‐CSF is a factor distinct from N‐CSF, that its production is dependent on the presence of sensitized T cells with antigen‐specific stimulation, and that it might be one of the causes of blood eosinophilia in this patient.
影响因子：
20.3
作者：
A. Burgess;D. Metcalf
通讯作者：
A. Burgess;D. Metcalf