Cyclooxygenase-2 Expression and Relationship to Malignant Potential in Human Bladder Cancer
Cyclooxygenase-2 Expression and Relationship to Malignant Potential in Human Bladder Cancer
复制标题
人类膀胱癌中环氧合酶 2 的表达及其与恶性潜能的关系
DOI：
10.1248/jhs.48.42
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发表时间：
2002
影响因子：
--
通讯作者：
S. Hara
中科院分区：
文献类型：
--
作者：
I. Matsuzawa;Y. Kondo;G. Kimura;Y. Hashimoto;S. Horie;N. Imura;M. Akimoto;S. Hara
环氧合酶（COX），催化花生四烯酸合成前列腺素，有两个同工型；COX-1和COX-2。大量证据表明COX-2在胃肠道癌症中很重要。为了确定COX-2是否在人膀胱移行细胞癌（TCC）和胃肠道癌中表达，我们通过逆转录聚合酶链反应（RT-PCR）和免疫组化分析考察了COX-2在人膀胱移行细胞癌中的表达，我们发现正常膀胱上皮不表达COX-2， COX-2在人膀胱TCC中明显上调。在非肿瘤组织中，COX-2免疫染色信号仅在淋巴滤泡中观察到。对膀胱癌组织中COX-2免疫染色的强度和程度进行评分，并探讨其与肿瘤分级和分期的关系。COX-2表达水平与肿瘤分级相关；从1级到3级，COX-2免疫染色评分逐步增加。这些发现提示COX-2表达的增加可能与膀胱癌和胃肠道癌的发生有关，并可能作为膀胱癌的生物标志物。
Cyclooxygenase (COX), which catalyzes the synthesis of prostaglandins from arachidonic acid, has two isoforms; COX-1 and COX-2. A large body of evidence exists to suggest that COX-2 is important in gastrointestinal cancer. In order to determine whether COX-2 is expressed in transitional cell carcinoma (TCC) of the human bladder as well as in gastrointestinal cancer, we investigated COX-2 expression in human TCC by Reverse Transcription-Polymerase Chain Reaction (RT-PCR) and immunohistochemical analysis, and we found that normal bladder epithelium did not express COX-2 and that COX-2 was markedly up-regulated in human bladder TCC. In nontumor tissues, COX-2 immunostaining signals were observed only in lymphoid follicles. Furthermore, the intensity and extent of COX-2 immunostaining in the bladder cancer tissues were scored and the relationship to tumor grade and stage was investigated. The levels of COX-2 expression were correlated with the tumor grade; from grades 1 to 3, there was a stepwise increase in the COX-2 immunostaining score. These findings suggested that an increase in COX-2 expression may be associated with bladder carcinogenesis as well as gastrointestinal carcinogenesis, and that it may be useful as a biomarker in bladder cancer.
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吡罗昔康对小鼠 OH-BBN 诱导的膀胱癌进行化学预防。
影响因子：
4.7
作者：
Moon,RC;Kelloff,GJ;Detrisac,CJ;Steele,VE;Thomas,CF;Sigman,CC
通讯作者：
Sigman,CC
影响因子：
11.2
作者：
H. Sheng;J. Shao;J. Morrow;R. Beauchamp;R. Dubois
通讯作者：
H. Sheng;J. Shao;J. Morrow;R. Beauchamp;R. Dubois
影响因子：
11.2
作者：
H. Sano;Y. Kawahito;R. Wilder;A. Hashiramoto;S. Mukai;K. Asai;S. Kimura;H. Kato;M. Kondo;T. Hla
通讯作者：
H. Sano;Y. Kawahito;R. Wilder;A. Hashiramoto;S. Mukai;K. Asai;S. Kimura;H. Kato;M. Kondo;T. Hla
阿司匹林对 N-[4-(5-硝基-2-呋喃基)-2-噻唑基]甲酰胺诱导的膀胱损伤的抑制与膀胱前列腺素内过氧化物合成酶的代谢抑制相关。
影响因子：
11.2
作者：
Cohen,SM;Zenser,TV;Murasaki,G;Fukushima,S;Mattammal,MB;Rapp,NS;Davis,BB
通讯作者：
Davis,BB