Guide Cells Support Muscle Regeneration and Affect Neuro-Muscular Junction Organization.
Guide Cells Support Muscle Regeneration and Affect Neuro-Muscular Junction Organization.
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引导细胞支持肌肉再生并影响神经肌肉接头组织。
DOI：
10.3390/ijms22041939
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发表时间：
2021-02-16
影响因子：
5.6
通讯作者：
Sampaolesi M
中科院分区：
文献类型：
--
作者：
Ronzoni FL;Giarratana N;Crippa S;Quattrocelli M;Cassano M;Ceccarelli G;Benedetti L;Van Herck J;Cusella De Angelis MG;Vitale M;Galli D;Sampaolesi M
肌肉再生是一个复杂的生物过程，发生在急性损伤和慢性退化过程中，涉及多种细胞类型。肌肉再生的最早事件之一是炎症反应，随后是肌肉祖细胞的激活和分化。然而，肌肉再生过程中新型神经肌肉接头形成的过程在很大程度上仍未被探索。在这里，我们通过单细胞 RNA 测序鉴定并分离出能够改善肌原性分化的血管相关细胞子集。我们将它们称为“引导”细胞，因为它们具有改善肌生成而不与新形成的纤维融合的卓越能力。在体外，这些细胞表现出显着的移动性和接触正在形成的肌管的能力。我们发现这些细胞的特征是 CD44 和 CD34 表面标记以及 Ng2 和 Ncam2 的表达。此外，在急性肌肉损伤和再生的小鼠模型中，注射引导细胞与新形成的神经肌肉接头数量的增加相关。因此，我们提出引导细胞在肌纤维再生过程中调节神经肌肉接头的从头生成。需要进一步的研究来调查这些细胞的起源以及再生肌纤维的终末规范所需的程度。
Muscular regeneration is a complex biological process that occurs during acute injury and chronic degeneration, implicating several cell types. One of the earliest events of muscle regeneration is the inflammatory response, followed by the activation and differentiation of muscle progenitor cells. However, the process of novel neuromuscular junction formation during muscle regeneration is still largely unexplored. Here, we identify by single-cell RNA sequencing and isolate a subset of vessel-associated cells able to improve myogenic differentiation. We termed them ‘guide’ cells because of their remarkable ability to improve myogenesis without fusing with the newly formed fibers. In vitro, these cells showed a marked mobility and ability to contact the forming myotubes. We found that these cells are characterized by CD44 and CD34 surface markers and the expression of Ng2 and Ncam2. In addition, in a murine model of acute muscle injury and regeneration, injection of guide cells correlated with increased numbers of newly formed neuromuscular junctions. Thus, we propose that guide cells modulate de novo generation of neuromuscular junctions in regenerating myofibers. Further studies are necessary to investigate the origin of those cells and the extent to which they are required for terminal specification of regenerating myofibers.
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影响因子：
3.7
作者：
Kim, Dae-Sung;Lee, Dongjin R.;Kim, Dong-Wook
通讯作者：
Kim, Dong-Wook
影响因子：
20.1
作者：
Leaf DE;Feig JE;Vasquez C;Riva PL;Yu C;Lader JM;Kontogeorgis A;Baron EL;Peters NS;Fisher EA;Gutstein DE;Morley GE
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Chauveau, Samuel;Brink, Peter R.;Cohen, Ira S.
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Cohen, Ira S.
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作者：
Entschladen, F;Drell, TL;Zaenker, KS
通讯作者：
Zaenker, KS
影响因子：
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作者：
Barbuti, Andrea;Galvez, Beatriz G.;DiFrancesco, Dario
通讯作者：
DiFrancesco, Dario