Multipurpose peptides: The venoms of Amazonian stinging ants contain anthelmintic ponericins with diverse predatory and defensive activities.
Multipurpose peptides: The venoms of Amazonian stinging ants contain anthelmintic ponericins with diverse predatory and defensive activities.
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DOI：
10.1016/j.bcp.2021.114693
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发表时间：
2021-10
影响因子：
5.8
通讯作者：
King, Glenn F.
中科院分区：
文献类型：
--
作者：
Nixon, Samantha A.;Robinson, Samuel D.;Agwa, Akello J.;Walker, Andrew A.;Choudhary, Shivani;Touchard, Axel;Undheim, Eivind A. B.;Robertson, Alan;Vetter, Irina;Schroeder, Christina I.;Kotze, Andrew C.;Herzig, Volker;King, Glenn F.
面对日益增加的耐药性，开发新的驱虫剂对于控制寄生在牲畜上的线虫至关重要。虽然海蜇毒液毒素在农业和医学上的应用引起了人们的注意，但很少有研究探索它们作为驱虫剂的潜力。在这里，我们评估了一个新的驱虫化合物的可能来源，通过筛选一个面板的10个hysteropteran毒液对捻转血矛线虫，反刍动物的主要病原线虫。利用生物活性导向分离技术结合液相色谱-串联质谱技术，从新热带区蚂蚁Neoponera apicalis毒液中分离鉴定了4种新的驱虫肽（ponericins）和一种新的驱虫肽（ponericin M-PONTX-Na 1b）。这些肽抑制H.捻转肌发育，1C 50值为2.8-5.6 μM。圆二色光谱表明，ponericin在水溶液中是非结构化的，而在模拟脂质环境中则是α-螺旋构象。我们发现，ponericins诱导非特异性膜扰动，赋予广谱抗菌，杀虫，细胞毒性，溶血和致藻活性，与所有检测的活性通常相关。我们还首次表明，ponericins诱导自发性疼痛行为时，注射在小鼠中。我们认为，ponericins的广谱活性使他们能够发挥捕食和防御作用的neoponteriants，符合其高丰度的毒液。这项研究揭示了一个更广泛的功能ponericins比以前假设的，并强调了机会和挑战，在追求蚂蚁毒液肽作为潜在的治疗。
In the face of increasing drug resistance, the development of new anthelmintics is critical for controlling nematodes that parasitise livestock. Although hymenopteran venom toxins have attracted attention for applications in agriculture and medicine, few studies have explored their potential as anthelmintics. Here we assessed hymenopteran venoms as a possible source of new anthelmintic compounds by screening a panel of ten hymenopteran venoms against Haemonchus contortus, a major pathogenic nematode of ruminants. Using bioassay-guided fractionation coupled with liquid chromatography-tandem mass spectromeuy, we identified four novel anthelmintic peptides (ponericins) from the venom of the neotropical ant Neoponera commutata and the previously described ponericin M-PONTX-Na1b from Neoponera apicalis venom. These peptides inhibit H. contortus development with 1C50 values of 2.8–5.6 μM. Circular dichroism spectropolarimeuy indicated that the ponericins are unstructured in aqueous solution but adopt α-helical conformations in lipid mimetic environments. We show that the ponericins induce non-specific membrane perturbation, which confers broad-specmuu antimicrobial, insecticidal, cytotoxic, hemolytic, and algogenic activities, with activity across all assays typically correlated. We also show for the first time that ponericins induce spontaneous pain behaviour when injected in mice. We propose that the broad spectrum activity of the ponericins enables them to play both a predato1y and defensive role in neoponeran ants, consistent with their high abundance in venom. This study reveals a broader functionality for ponericins than previously assumed, and highlights both the opportunities and challenges in pursuing ant venom peptides as potential therapeutics.
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DOI：
10.1073/pnas.1307010110
发表时间：
2013-08-27
影响因子：
11.1
作者：
Lee, Ming-Tao;Sun, Tzu-Lin;Huang, Huey W.
通讯作者：
Huang, Huey W.
影响因子：
2.6
作者：
ALBERS, GAA;BURGESS, SK
通讯作者：
BURGESS, SK
影响因子：
4.7
作者：
Agwa, Akello J.;Blomster, Linda, V;Schroeder, Christina, I
通讯作者：
Schroeder, Christina, I
色氨酸在鸭抗菌肽 dCATH 抗菌活性和细胞毒性中的关键作用
影响因子：
5.2
作者：
Feng, Xingjun;Jin, Sanjun;Liu, Yueying
通讯作者：
Liu, Yueying
影响因子：
2.3
作者：
LeBrun, Edward G.;Diebold, Peter J.;Gilbert, Lawrence E.
通讯作者：
Gilbert, Lawrence E.