Synaptic circuitry of identified neurons in the antennal lobe of Drosophila melanogaster.
Synaptic circuitry of identified neurons in the antennal lobe of Drosophila melanogaster.
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DOI：
10.1002/cne.23966
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发表时间：
2016-06-15
期刊：
影响因子：
--
通讯作者：
Hansson BS
中科院分区：
文献类型：
--
作者：
Rybak J;Talarico G;Ruiz S;Arnold C;Cantera R;Hansson BS
黑腹果蝇嗅感觉神经元（OSNs）在触角叶（AL）肾小球内与投射神经元（PNs）和局部中间神经元建立突触。功能性研究已经获得了关于这种电路的大量知识，而突触接触的超微结构证据却很少。为了填补这一空白，我们用电子显微镜研究了三个肾小球的连续切片。膜结合过氧化物酶的异位表达使我们能够沿着PN树突绘制突触位点。我们的数据首次证明，三种主要类型的AL神经元中的每一种都是突触前和突触后的其他两种类型，正如之前的功能研究所表明的那样。PN树突携带很大比例的输出突触，大约每三个输入突触有一个输出。PN树突的详细重建显示，这些突触分布不均匀，输入和输出位点沿着近端和远端梯度部分分离，最薄的分支仅携带输入突触。此外，我们的数据表明突触聚集，因为我们发现了PN树突树突平铺的证据。PN输出突触的突触前密度呈T形，多数呈四分体排列。相比之下，来自假设的神经网络的输出突触显示出延长的突触前密度，其中T - bar平台由几个基座支撑，并接触多达20个突触后轮廓。我们还发现了假定的osn之间的突触接触。肾小球神经细胞的平均突触密度约为2个突触/µm3。这些结果讨论了关于嗅觉肾小球微电路跨物种的当前模型。[j] .中国生物医学工程学报，2016。©2016作者比较神经病学杂志由Wiley期刊公司出版。
In Drosophila melanogaster olfactory sensory neurons (OSNs) establish synapses with projection neurons (PNs) and local interneurons within antennal lobe (AL) glomeruli. Substantial knowledge regarding this circuitry has been obtained by functional studies, whereas ultrastructural evidence of synaptic contacts is scarce. To fill this gap, we studied serial sections of three glomeruli using electron microscopy. Ectopic expression of a membrane‐bound peroxidase allowed us to map synaptic sites along PN dendrites. Our data prove for the first time that each of the three major types of AL neurons is both pre‐ and postsynaptic to the other two types, as previously indicated by functional studies. PN dendrites carry a large proportion of output synapses, with approximately one output per every three input synapses. Detailed reconstructions of PN dendrites showed that these synapses are distributed unevenly, with input and output sites partially segregated along a proximal–distal gradient and the thinnest branches carrying solely input synapses. Moreover, our data indicate synapse clustering, as we found evidence of dendritic tiling of PN dendrites. PN output synapses exhibited T‐shaped presynaptic densities, mostly arranged as tetrads. In contrast, output synapses from putative OSNs showed elongated presynaptic densities in which the T‐bar platform was supported by several pedestals and contacted as many as 20 postsynaptic profiles. We also discovered synaptic contacts between the putative OSNs. The average synaptic density in the glomerular neuropil was about two synapses/µm3. These results are discussed with regard to current models of olfactory glomerular microcircuits across species. J. Comp. Neurol. 524:1920–1956, 2016. © 2016 The Authors The Journal of Comparative Neurology Published by Wiley Periodicals, Inc.
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