Coaction of spheroid-derived stem-like cells and endothelial progenitor cells promotes development of colon cancer.
Coaction of spheroid-derived stem-like cells and endothelial progenitor cells promotes development of colon cancer.
复制标题
球状体干细胞与内皮祖细胞的相互作用促进结肠癌的发展
DOI：
10.1371/journal.pone.0039069
复制
发表时间：
2012
期刊：
影响因子：
3.7
通讯作者：
Wei HB
中科院分区：
文献类型：
--
作者：
Wei B;Han XY;Qi CL;Zhang S;Zheng ZH;Huang Y;Chen TF;Wei HB
Although some studies described the characteristics of colon cancer stem cells (CSCs) and the role of endothelial progenitor cells (EPCs) in neovascularization, it is still controversial whether an interaction exists or not between CSCs and EPCs. In the present study, HCT116 and HT29 sphere models, which are known to be the cells enriching CSCs, were established to investigate the roles of this interaction in development and metastasis of colon cancer. Compared with their parental counterparts, spheroid cells demonstrated higher capacity of invasion, higher tumorigenic and metastatic potential. Then the in vitro and in vivo relationship between CSCs and EPCs were studied by using capillary tube formation assay and xenograft models. Our results showed that spheroid cells could promote the proliferation, migration and tube formation of EPCs through secretion of vascular endothelial growth factor (VEGF). Meanwhile, the EPCs could increase tumorigenic capacity of spheroid cells through angiogenesis. Furthermore, higher microvessel density was detected in the area enriching cancer stem cells in human colon cancer tissue. Our findings indicate that spheroid cells possess the characteristics of cancer stem cells, and the coaction of CSCs and EPCs may play an important role in the development of colon cancer.
登录
查看更多内容
影响因子：
56.9
作者：
Asahara, T;Murohara, T;Isner, JM
通讯作者：
Isner, JM
影响因子：
6.4
作者：
Hasan, Mohammad R.;Ho, Shirley H. Y.;Tai, Isabella T.
通讯作者：
Tai, Isabella T.
影响因子：
7.3
作者：
Burkert, J.;Otto, W. R.;Wright, N. A.
通讯作者：
Wright, N. A.
影响因子：
8.8
作者：
通讯作者：
--
影响因子：
11.2
作者：
Li, Chenwei;Heidt, David G.;Simeone, Diane M.
通讯作者：
Simeone, Diane M.