Separation of protein oligomers by blue native gel electrophoresis.
Separation of protein oligomers by blue native gel electrophoresis.
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DOI:
10.1016/j.ab.2009.02.019
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发表时间:
2009-05-01
影响因子:
2.9
通讯作者:
Howard KJ
中科院分区:
文献类型:
--
作者:
Braz VA;Howard KJ
Native gel electrophoresis is used as a tool to assess structural differences in proteins. This note presents an application to separate oligomeric forms of proteins, such as HIV-1 reverse transcriptase monomers and homodimers. Technical difficulties encountered with various native gel techniques and ways to circumvent them are described.
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